Hamster Anti-Mouse CD28-FITC

Cat. No.
1610-02  
Size
0.5 mg  
Price (USD)
$225.00 
Clone 37.51
Isotype Hamster (Syrian) IgG2
Isotype Control Hamster IgG-FITC
Immunogen C57BL/6N mouse T cell lymphoma EL-4 cell line
Specificity Mouse CD28
Alternalte Name(s) Tp44, T44
Description CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2
Immunoprecipitation – Reported in literature 1
Costimulation – Reported in literature 1-3,7-9,11
Stimulation – Reported in literature 10
Activation – Reported in literature 5
Blocking – Reported in literature 6
Recommended Dilutions Please refer to product specific Technical Bulletin
  • BALB/c mouse thymocytes were stained with Hamster Anti-Mouse CD28-FITC (SB Cat. No. 1610-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).

BALB/c mouse thymocytes were stained with Hamster Anti-Mouse CD28-FITC (SB Cat. No. 1610-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
 
References
1. Gross JA, Callas E, Allison JP. Identification and distribution of the costimulatory receptor CD28 in mouse. J Immunol. 1992;149:380-8. (Immunogen, FC, IP, Costim)
2. Silverio JC, Pereira IR, Cipitelli M, Vinagre NF, Rodrigues MM, Gazzinelli RT, et al. CD8+ T-cells expressing interferon gamma or perforin play antagonistic roles in heart injury in experimental Trypanosoma cruzi-elicited cardiomyopathy. PLoS Pathog. 2012;8(4):e1002645. (Costim)
3. Lin J, Harding A, Giurisato E, Shaw AS. KSR1 modulates the sensitivity of mitogen-activated protein kinase pathway activation in T cells without altering fundamental system outputs. Mol Cell Biol. 2009;29:2082-91. (Costim)
4. Nandi D, Gross JA, Allison JP. CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells. J immunol. 1994;152:3361-9. (FC, Costim)
5. Cibotti R, Punt JA, Dash KS, Sharrow SO, Singer A. Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals. Immunity. 1997;6:245-55. (Activ)
6. Nishio M, Spielman J, Lee RK, Nelson DL, Podack ER. CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis. J Immunol. 1996;157:4347-53. (Block)
7. Kim EH, Sullivan JA, Plisch EH, Tejera MM, Jatzek A, Choi KY, et al. Signal integration by Akt regulates CD8 T cell effector and memory differentiation. J Immunol. 2012;188:4305-14. (Costim)
8. Yoshida K, Sakamoto A, Yamashita K, Arguni E, Horigome S, Arima M, et al. Bcl6 controls granzyme B expression in effector CD8+ T cells. Eur J Immunol. 2006;36:3146-56. (Costim)
9. Belhacéne N, Gamas P, Gonçalvès D, Jacquin M, Beneteau M, Jacquel A, et al. Severe thymic atrophy in a mouse model of skin inflammation accounts for impaired TNFR1 signaling. PLoS One. 2012;7(10):e47321. (Costim)
10. Zhang T, Fresnay S, Welty E, Sangrampurkar N, Rybak E, Zhou H, et al. Selective CD28 blockade attenuates acute and chronic rejection of murine cardiac allografts in a CTLA-4-dependent manner. Am J Transplant. 2011;11:1599-1609. (Stim)
11. Shen XZ, Okwan-Doudu D, Blackwell W, Ong FS, Janjulia T, Bernstein EA. Myeloid expression of angiotensin-converting enzyme facilitates myeloid maturation and inhibits the development of myeloid-derived suppressor cells. Lab Invest. 2014;94:536-44. (Costim)