Rat Anti-Mouse CD16/32-PE/CY5.5

Cat. No.
1630-16  
Size
0.1 mg  
Price (USD)
$195.00 
Clone 93
Isotype Rat IgG2aλ
Isotype Control Rat IgG2a-PE/CY5.5
Immunogen VH81X Tg B cells and T220 stromal cell line
Specificity Mouse CD16/32
Alternalte Name(s) FcRγII, FcRγIII, Fc gamma II receptor, Fc gamma III receptor, FcFR2, IGFR2, FcGR3, IGFR3, Fc receptor block
Description The lymphocyte Fcγ receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fcγ type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying FcγR+ cells, monoclonal antibodies to the FcγII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on FcγR for parasitic invasion. The monoclonal antibody 93 recognizes a conformational epitope formed by FcγII and FcγIII receptors.
Format/Conjugate PE/CY5.5 (R-phycoerythrin/Cyanine 5.5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2,5
Immunohistochemistry-Frozen Sections – Reported in literature 6
Immunoprecipitation – Reported in literature 6
Blocking – Reported in literature 1-4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795062
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD16/32-PE/CY5.5 (SB Cat. No. 1630-16) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD16/32-PE/CY5.5 (SB Cat. No. 1630-16) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).
 
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References
1. Rainczuk A, Scorza T, Smooker PM, Spithill TW. Induction of specific T-cell responses, opsonizing antibodies, and protection against Plasmodium chabaudi adami infection in mice vaccinated with genomic expression libraries expressed in targeted and secretory DNA vectors. Infect Immun. 2003;71:4506-15. (Block)
2. Petersen LK, Xue L, Wannemuehler MJ, Rajan K, Narasimhan B. The simultaneous effect of polymer chemistry and device geometry on the in vitro activation of murine dendritic cells. Biomaterials. 2009;30:5131-42. (Block, FC)
3. Safari D, Dekker HA, Rijkers G, Snippe H. Codelivery of adjuvants at the primary immunization site is essential for evoking a robust immune response to neoglycoconjugates. Vaccine. 2011;29:849-54. (Block)
4. Petersen LK, Ramer-Tait AE, Broderick SR, Kong C, Ulery BD, Rajan K, et al. Activation of innate immune responses in a pathogen-mimicking manner by amphiphilic polyanhydride nanoparticle adjuvants. Biomaterials. 2011;32:6815-22. (Block)
5. He W, Hu Z, Liu F, Feng X, Zou P. In vitro co-stimulation of anti-tumor activity by soluble B7 molecules. Acta Biochim Pol. 2006;53:807-13. (FC)
6. Personal communication (IHC-FS, IP)
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