Goat Anti-Mouse IgG, Human ads-BIOT

Cat. No.
1030-08  
Size
1.0 mg  
Price (USD)
$100.00 
Specificity Reacts with the heavy chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Mouse IgM and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-10
FLISA
Flow Cytometry 5,11-13
Other referenced applications for relevant formats include -
ELISpot 1,3-6
Immunohistochemistry-Frozen Sections 2,14,15
Immunohistochemistry-Paraffin Sections 16-18
Immunocytochemistry 19-22
Western Blot 7,21,23-25
Multiplex 26,27
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.

ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
 
References
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