Mouse Anti-Biotin-FITC (SB58c)
FITC Anti-Biotin antibody for use in flow cytometry and western blot assays.
|Isotype||Mouse (BALB/c) IgG1κ|
|Isotype Control||Mouse IgG1-FITC (15H6)|
|Description||Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives.|
|Buffer Formulation||Phosphate buffered saline containing < 0.1% sodium azide|
|Recommended Storage||2-8°C; Avoid exposure to light|
|Applications|| ELISA – Quality tested|
FLISA – Quality tested
Flow Cytometry – Quality tested
Western Blot – Reported in literature 1
Proximity Ligation Assay – Reported in literature 2
- 1. Späte A, Schart VF, Häfner J, Niederwieser A, Mayer TU, Wittmann V. Expanding the scope of cyclopropene reporters for the detection of metabolically engineered glycoproteins by Diels-Alder reactions. Beilstein J Org Chem. 2014;10:2235-42. (WB)
- 2. Workman ER, Haddick PC, Bush K, Dilly GA, Niere F, Zemelman BV, et al. Rapid antidepressants stimulate the decoupling of GABAB receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3η. Mol Psychiatry. 2015;20:298-310. (Proximity ligation assay)
- 3. Fahie MA, Yang B, Mullis M, Holden MA, Chen M. Selective detection of protein homologues in serum using an OmpG nanopore. Anal Chem. 2015;87:11143-9.