Goat Anti-Type IV Collagen-UNLB

Cat. No.
1340-01  
Size
0.2 mg  
Price (USD)
$395.00 
Specificity Reacts with conformational determinants on type IV collagen
Source Pooled antisera from goats hyperimmunized with type IV collagen
Cross Adsorption Collagen types I, II, III, V, and VI
Purification Affinity chromatography on type IV collagen covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.4 mg/mL
Volume 0.5 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Paraffin Sections 2-12
Immunohistochemistry-Frozen Sections 6,13-28
Immunocytochemistry 6,14,27,29,30
Electron Microscopy 5,31,32
Flow Cytometry 36
Western Blot 2,4,7,8,10,14,27,29,32,33
Immunoprecipitation 32-35
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2721907
  • Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
  • Frozen sections of E12.5 eyes from Col4a1+/+ mice were stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a secondary antibody and DAPI.

    Image from Mao M, Kiss M, Ou Y, Gould DB. Genetic dissection of anterior segment dysgenesis caused by a Col4a1 mutation. Dis Model Mech. 2017 Feb 24. doi: 10.1242/dmm.027888. [Epub ahead of print]. Figure S6(a)
  • Frozen Itga3-/-/Gpr56-/- E11.5 mouse neocortex section was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01), anti-Tuj1, and anti-Nestin followed by secondary antibodies.

    Image from Jeong S, Luo R, Singer K, Giera S, Kreidberg J, Kiyozumi D, et al. GPR56 functions together with α3β1 integrin in regulating cerebral cortical development. PLoS One. 2013;8(7):e68781. Figure 4(h)
  • Frozen human nasal mucosa explant section 24 h post infection with HSV1 was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) and anti-HSV1 gD followed by a secondary reagents.

    Image from Glorieux S, Bachert C, Favoreel HW, Vandekerckhove AP, Steukers L, Rekecki A, et al. Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa. PLoS One. 2011;6(7):e22160. Figure 4(b)
  • Frozen C57BL/6 mouse quadricep muscle section was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a secondary antibody.

    Image from Wehling-Henricks M, Jordan MC, Gotoh T, Grody WW, Roos KP, Tidball JG. Arginine metabolism by macrophages promotes cardiac and muscle fibrosis in mdx muscular dystrophy. PloS One. 2010;5(5):e10763. Figure 4
  • Frozen rat kidney section following SNX was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a secondary antibody and DAB.

    Image from Yuen DA, Connelly KA, Advani A, Liao C, Kuliszewski MA, Trogadis J, et al. Culture-modified bone marrow cells attenuate cardiac and renal injury in a chronic kidney disease rat model via a novel antifibrotic mechanism. PLoS One. 2010;5(3):e9543. Figure 3(b)
  • Paraffin embedded rat kidney section was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a HRP conjugated secondary antibody and DAB.

    Image from Kawamorita Y, Shiraishi T, Tamura Y, Kumagai T, Shibata S, Fujigaki Y, et al. Renoprotective effect of topiroxostat via antioxidant activity in puromycin aminonucleoside nephrosis rats. Physiol Rep. 2017;5:e13358. Figure 2(e)

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
 

Frozen sections of E12.5 eyes from Col4a1+/+ mice were stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a secondary antibody and DAPI.

Image from Mao M, Kiss M, Ou Y, Gould DB. Genetic dissection of anterior segment dysgenesis caused by a Col4a1 mutation. Dis Model Mech. 2017 Feb 24. doi: 10.1242/dmm.027888. [Epub ahead of print]. Figure S6(a)
 

Frozen Itga3-/-/Gpr56-/- E11.5 mouse neocortex section was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01), anti-Tuj1, and anti-Nestin followed by secondary antibodies.

Image from Jeong S, Luo R, Singer K, Giera S, Kreidberg J, Kiyozumi D, et al. GPR56 functions together with α3β1 integrin in regulating cerebral cortical development. PLoS One. 2013;8(7):e68781. Figure 4(h)
 

Frozen human nasal mucosa explant section 24 h post infection with HSV1 was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) and anti-HSV1 gD followed by a secondary reagents.

Image from Glorieux S, Bachert C, Favoreel HW, Vandekerckhove AP, Steukers L, Rekecki A, et al. Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa. PLoS One. 2011;6(7):e22160. Figure 4(b)
 

Frozen C57BL/6 mouse quadricep muscle section was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a secondary antibody.

Image from Wehling-Henricks M, Jordan MC, Gotoh T, Grody WW, Roos KP, Tidball JG. Arginine metabolism by macrophages promotes cardiac and muscle fibrosis in mdx muscular dystrophy. PloS One. 2010;5(5):e10763. Figure 4
 

Frozen rat kidney section following SNX was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a secondary antibody and DAB.

Image from Yuen DA, Connelly KA, Advani A, Liao C, Kuliszewski MA, Trogadis J, et al. Culture-modified bone marrow cells attenuate cardiac and renal injury in a chronic kidney disease rat model via a novel antifibrotic mechanism. PLoS One. 2010;5(3):e9543. Figure 3(b)
 

Paraffin embedded rat kidney section was stained with Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01) followed by a HRP conjugated secondary antibody and DAB.

Image from Kawamorita Y, Shiraishi T, Tamura Y, Kumagai T, Shibata S, Fujigaki Y, et al. Renoprotective effect of topiroxostat via antioxidant activity in puromycin aminonucleoside nephrosis rats. Physiol Rep. 2017;5:e13358. Figure 2(e)
 
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References
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