Mouse Anti-Mouse Bax-UNLB (5B7)
Cat. No.:
10050-01
Purified Anti-Mouse Bax antibody for use in flow cytometry, immunocytochemistry, western blot, immunoprecipitation, and purification assays.
$198.00
Clone | 5B7 |
---|---|
Isotype | Mouse IgG1κ |
Isotype Control | Mouse IgG1-UNLB (15H6) |
Specificity | Mouse Bax |
Alternative Names | Apoptosis regulator BAX, BCL2 associated X protein |
Description | Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 5B7 recognizes both native and activated forms of Bax as well as Bax-Bcl-2 and Bax-Bcl-xL heterodimers. |
Immunogen | Murine Bax peptide (a.a. 3-16) |
Conjugate | UNLB (Unconjugated) |
Buffer Formulation | Borate buffered saline, pH 8.2 |
Clonality | Monoclonal |
Concentration | 0.1 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C |
Applications |
Western Blot – Quality tested 1-3,5 Immunoprecipitation – Reported in literature 1,3,5 Flow Cytometry – Reported in literature 4 Immunocytochemistry – Reported in literature 4 Purification – Reported in literature 5 |
RRID Number | AB_2794106 |
Gene ID |
12028 (Mouse) |
Gene ID Symbol |
Bax (Mouse) |
UniProt ID |
Q07813 (Mouse) |
UniProt Name |
BAX_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, WB, IP)
- 2. Hsu Y, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-XL during apoptosis. Proc Natl Acad Sci USA. 1997;94:3668-72. (WB)
- 3. Havasi A, Li Z, Wang Z, Martin JL, Botla V, Ruchalski K, et al. Hsp27 inhibits Bax activation and apoptosis via a phosphatidylinositol 3-kinase-dependent mechanism. J Biol Chem. 2008;283:12305-13. (WB, IP)
- 4. Halicka HD, Smolewski P, Darzynkiewicz Z, Dai W, Traganos F. Arsenic trioxide arrests cells early in mitosis leading to apoptosis. Cell Cycle. 2002;1:200-8. (FC, ICC)
- 5. Hsu Y, Youle RJ. Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J Biol Chem. 1998;273:10777-83. (WB, IP, Purification)
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