Rat Anti-Mouse CD45-LE/AF

Cat. No.
1660-14  
Size
0.5 mg  
Price (USD)
$250.00 
Clone I3/2.3
Isotype Rat (Lewis) IgG2bλ
Isotype Control Rat IgG2b-LE/AF
Immunogen T1M1 (Thy-1c) cells
Specificity Mouse CD45
Alternalte Name(s) LCA, leukocyte common antigen, T200, Ly-5, Receptor-type tyrosine-protein phosphatase C, Lyt-4, Ptprc
Description CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2,5-7
Immunohistochemistry-Frozen Sections – Reported in literature 3,4
Immunoprecipitation – Reported in literature 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-PE/TXRD (SB Cat. No. 1660-10).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-PE/TXRD (SB Cat. No. 1660-10).
 
References
1. Trowbridge IS. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. J Exp Med. 1978;148:313-23. (Immunogen, IP)
2. Haidl ID, Ng DH, Rothenberger S, Johnson P, Jefferies WA. Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells. Eur J Immunol. 1995;25:3370-4. (FC, IP)
3. Craner MJ, Damarjian TG, Liu S, Hains BC, Lo AC, Black JA, et al. Sodium channels contribute to microglia/macrophage activation and function in EAE and MS. Glia. 2005;49:220-9. (IHC-FS)
4. Villalta SA, Lang J, Kubeck S, Kabre B, Szot GL, Calderon B, et al. Inhibition of VEGFR-2 reverses type 1 diabetes in NOD mice by abrogating insulitis and restoring islet function. Diabetes. 2013;62:2870-8. (IHC-FS)
5. Takedachi M, Qu D, Ebisuno Y, Oohara H, Joachims ML, McGee ST, et al. CD73-generated adenosine restricts lymphocyte migration into draining lymph nodes. J Immunol. 2008;180:6288-96. (FC)
6. Matsunaga H, Hokari R, Kurihara C, Okada Y, Takebayashi K, Okudaira K, et al. Omega-3 fatty acids exacerbate DSS-induced colitis through decreased adiponectin in colonic subepithelial myofibroblasts. Inflamm Bowel Dis. 2008;14:1348-57. (FC)
7. Domingos-Pereira S, Decrausaz L, Derré L, Bobst M, Romero P, Schiller JT, et al. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol. 2013;6:393-404. (FC)