Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Rat Anti-Mouse CD45-AF700 (I3/2.3)

Cat. No.:
Alexa Fluor® 700 Anti-Mouse CD45 antibody for use in flow cytometry assays.
Size Price (USD) Quantity
0.1 mg $219.00
More Information
Clone I3/2.3
Isotype Rat (Lewis) IgG2bλ
Isotype Control Rat IgG2b-AF700 (KLH/G2b-1-2)
Specificity Mouse CD45
Alternative Names LCA, leukocyte common antigen, T200, Ly-5, Receptor-type tyrosine-protein phosphatase C, Lyt-4, Ptprc
Description CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.
Immunogen T1M1 (Thy-1c) cells
Conjugate AF700 (Alexa Fluor® 700)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 2,5-7
Immunohistochemistry-Frozen Sections – Reported in literature 3,4
Immunoprecipitation – Reported in literature 1,2

RRID Number AB_2795109
Gene ID 19264 (Mouse)
Gene ID Symbol Ptprc (Mouse)
Gene ID Aliases loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
UniProt ID P06800 (Mouse
UniProt Name PTPRC_MOUSE (Mouse)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

Need help with this product?
or call 800.722.2255
  1. 1. Trowbridge IS. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. J Exp Med. 1978;148:313-23. (Immunogen, IP)
  2. 2. Haidl ID, Ng DH, Rothenberger S, Johnson P, Jefferies WA. Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells. Eur J Immunol. 1995;25:3370-4. (FC, IP)
  3. 3. Craner MJ, Damarjian TG, Liu S, Hains BC, Lo AC, Black JA, et al. Sodium channels contribute to microglia/macrophage activation and function in EAE and MS. Glia. 2005;49:220-9. (IHC-FS)
  4. 4. Villalta SA, Lang J, Kubeck S, Kabre B, Szot GL, Calderon B, et al. Inhibition of VEGFR-2 reverses type 1 diabetes in NOD mice by abrogating insulitis and restoring islet function. Diabetes. 2013;62:2870-8. (IHC-FS)
  5. 5. Takedachi M, Qu D, Ebisuno Y, Oohara H, Joachims ML, McGee ST, et al. CD73-generated adenosine restricts lymphocyte migration into draining lymph nodes. J Immunol. 2008;180:6288-96. (FC)
  6. 6. Matsunaga H, Hokari R, Kurihara C, Okada Y, Takebayashi K, Okudaira K, et al. Omega-3 fatty acids exacerbate DSS-induced colitis through decreased adiponectin in colonic subepithelial myofibroblasts. Inflamm Bowel Dis. 2008;14:1348-57. (FC)
  7. 7. Domingos-Pereira S, Decrausaz L, Derré L, Bobst M, Romero P, Schiller JT, et al. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol. 2013;6:393-404. (FC)
See All References