The Antibody Company - SouthernBiotech
 
   

Fluoromount-G®

Cat. No.
0100-01  
Size
25 mL  
Price (USD)
$35.00 
Qty
Add to Cart
Description Fluoromount-G® is a water-soluble compound recommended for slides mounted after a staining procedure having an aqueous final step. Since Fluoromount-G® is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened. This mounting medium also provides a semi-permanent seal for storage of slide preparations.
Buffer Formulation Supplied with NaN3 as a preservative
Volume 25.0 mL
Storage & Handling Store at 22-25°C
Please refer to product specific SDS
Applications Immunohistochemistry-Frozen Sections 1-28
Immunohistochemistry-Paraffin Sections 7,29,30
Immunohistochemistry-Whole Mount 31-33,48
Immunocytochemistry 12,17,30,34-64
In situ Hybridization 6,65
TUNEL 6,54
Proximity Ligation Assay 66
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G (SB Cat. No. 0100-01).

    Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)
  • Zika virus (ZIKV) inoculated frozen mouse placenta was stained with anti-flaviviruses and anti-cytokeratin. The DNA was stained with DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Vermillion MS, Lei J, Shabi Y, Baxter VK, Crilly NP, McLane M, et al. Intrauterine Zika virus infection of pregnant immunocompetent mice models transplacental transmission and adverse perinatal outcomes. Nat Commun. 2017;8:14575. Figure 4(b)
  • Frozen zebrafish retina section was stained with anti-Zpr3 followed by an AF543 secondary antibody and MBODEC and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Watanabe K, Nishimura Y, Oka T, Nomoto T, Kon T, Shintou T, et al. In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neurosci. 2010;11:116. Figure 3(d)
  • Whole mount Ophryotrocha diadema adult was stained with TRITC conjugated phalliodin and anti-acetylated tubulin followed by a CY2 conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Bergter A, Brubacher JL, Paululat A. Muscle formation during embryogenesis of the polychaete Ophryotrocha diadema (Dorvilleidae) - new insights into annelid muscle patterns. Front Zool. 2008;5:1. Figure 2(a)
  • Whole mount Lineus albocinctus pilidium larva was stained with anti-FMRFamide and anti-acetylated- α-tubulin followed by AF568 and AF633 conjugated secondary antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Hindinger S, Schwaha T, Wanninger A. Immunocytochemical studies reveal novel neural structures in nemertean pilidium larvae and provide evidence for incorporation of larval components into the juvenile nervous system. Front Zool. 2013;10:31. Figure 5(a)
  • Optic nerve explant cultures were stained with anti-Stim2 and anti-GPAF followed by secondary antibodies. The DNA was stained with Hoechst and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Papanikolaou M, Lewis A, Butt AM. Store-operated calcium entry is essential for glial calcium signalling in CNS white matter. Brain Struct Funct. 2017 Feb 28. doi:10.1007/s00429-017-1380-8 [Epub ahead of print]. Figure 3(c)
  • Frozen simian immunodeficiency virus infected rhesus macaque ileum section was stained with anti-IL-7 and anti-cytokeratin followed by AF488 and AF546 conjugated secondary antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Ponte R, Rancez M, Figueiredo-Morgado S, Dutrieux J, Fabre-Mersseman V, Charmeteau-de-Muylder B, et al. Acute simian immunodeficiency virus infection triggers early and transient interleukin-7 production in the gut, leading to enhanced local chemokine expression and intestinal immune cell homing. Front Immunol. 2017;8:588. Figure 1(d)
  • Whole mount Symsagittifera roscoffensis embryo was stained with AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool. 2008;5:14. Figure 2(b)
  • pEGP-BDNF transfected human umbilical cord blood-derived mesenchymal stem cells were stained with anti-BDNF followed by a secondary antibody and DAB and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Lim JY, Park SH, Jeong CH, Oh JH, Kim SM, Ryu CH, et al. Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells. BMC Biotechnol. 2010;10:38. Figure 7(a)
  • Twistp-GFP-actin-Vrp1f06715 transfected porcine aortic endothelial cells were stained with TRITC conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Eriksson T, Varshney G, Aspenström P, Palmer RH. Characterisation of the role of Vrp1 in cell fusion during the development of visceral muscle of Drosophila melanogaster. BMC Dev Biol. 2010;10:86. Figure 8(b)
  • Cultured rat retinal ganglion cells were stained with anti-Thy-1 and anti-neurofilament-L followed by secondary antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Pang I, Zeng H, Fleenor DL, Clark AF. Pigment epithelium-derived factor protects retinal ganglion cells. BMC Neurosci. 2007;8:11. Figure 1(d)
  • Whole mount Hispolia malayensis zooid was stained with an AF488 conjugated phalloidin followed by DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Schwaha T, Wood TS, Wanninger A. Myoanatomy and serotonergic nervous system of the ctenostome Hislopia malayensis: evolutionary trends in bodyplan patterning of ectoprocta. Front Zool. 2011;8:11. Figure 3(b)
  • Arabidopsis culture following BrdU incubation was stained with anti-BrdU followed by an AF488 conjugated secondary antibody and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Kotogány E, Dudits D, Horváth GV, Ayaydin F. A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine. Plant Methods. 2010;6:5. Figure 1(b)
  • Human retinal pigmented epithelial cells were stained with 546 labeled phalloidin and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Hua K, Ferland RJ. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5. Figure 1
  • SLICK-H::Ranbp2flox/flox YFP-expressing mouse nerve cyrosections at day10 post-tamoxifen administration were stained with anti-HDAC4 followed by an AF594 conjugated secondary antibody, Hoechst 33342, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Cho K, Yoon D, Qiu S, Danziger Z, Grill WM, Wetsel WC, et al. Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes. Dis Model Mech. 2017;10:559-79. Figure 4(d)
  • MDA-MB-231 cells were stained with conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from McConnell BV, Koto K, Gutierrez-Hartmann A. Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression. Mol Cancer. 2011;10:75. Figure 5
  • Frozen I4895T mouse soleus muscle section was stained with PROTEOSTAT® aggresome detection reagent and Hoechst 33342 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Lee CS, Hanna AD, Wang H, Dagnino-Acosta A, Joshi AD, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659. Figure 6(a)

    PROTEOSTAT® is a registered trademark of Enzo Life Sciences, Inc.
  • Human retinal pigmented epithelial cells were stained with anti-β-tubulin followed by an AF488 secondary antibody and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Hua K, Ferland RJ. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5. Figure 1
  • Whole mount Ophryotrocha diadema embryo was stained with TRITC conjugated phalliodin and anti-acetylated tubulin followed by a CY2 conjugated secondary antibody and DRAQ5™ and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Bergter A, Brubacher JL, Paululat A. Muscle formation during embryogenesis of the polychaete Ophryotrocha diadema (Dorvilleidae) - new insights into annelid muscle patterns. Front Zool. 2008;5:1. Figure 5(g)

    DRAQ5™ is a registered trademark of Biostatus Limited
  • Frozen mouse circumvallate papillae section was stained with anti-IP3R3 followed by a Rhodamine Red™-X secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Clapp TR, Stone LM, Margolskee RF, Kinnamon SC. Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction. BMC Neurosci. 2001;2:6. Figure 4(b)

    Rhodamine Red™ is a trademark of Thermo Fisher Scientific, Inc.
  • Frozen Dcc+/+ mouse lumbar dorsal root ganglion section was stained with anti-CGRP, anti-isolectin B4, and anti-Substance P followed by AF488, AF555, and AF647 conjugated secondary reagents and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Liang D, Zheng M, Sun Y, Sahbaie P, Low SA, Peltz G, et al. The Netrin-1 receptor DCC is a regulator of maladaptive responses to chronic morphine administration. BMC Genomics. 2014;15:345. Figure 5(a)
  • Whole mount Symsagittifera roscoffensis embryo was stained with AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool. 2008;5:14. Figure 3(a)
  • Frozen TRPM5-GFP mouse circumvallate papillae section was stained with propidium iodide and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Vandenbeuch A, Clapp TR, Kinnamon SC. Amiloride-sensitive channels in type I fungiform taste cells in mouse. BMC Neurosci. 2008;9:1. Figure 5
  • Whole mount Drosophila larva testis was stained with anti-DSXM, anti-TJ, and anti-EYA followed by AF488 and AF647 conjugated secondary antibodies and tyramide signal amplification CY3 system and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Hempel LU, Oliver B. Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells. BMC Dev Biol. 2007;7:113. Figure 7(d)
  • Whole S. littoralis ovariole was stained with anti-YP2 followed by an AF594 conjugated secondary antibody, Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Bebas P, Kotwica J, Joachimiak E, Giebultowicz JM. Yolk protein is expressed in the insect testis and interacts with sperm. BMC Dev Biol. 2008;8:64. Figure 4(b)
  • Frozen mouse embryo jugular lymph sac section was stained with anti-Prox1 and anti-LA102 followed by AF488 and AF594 conjuagted secondary antibodies and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Buttler K, Ezaki T, Wilting J. Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics. BMC Dev Biol. 2008;8:43. Figure 1(d)
  • Frozen bitransgenic MTB/TRAS mouse mammary tissue section was stained with anti-cytokeratin 8 and anti-Smad4 followed by AF488 and AF567 conjugated secondary antibodies and Hoechst 33258 then mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Liu Z, Wang M, Alvarez JV, Bonney ME, Chen C, D'Cruz C, et al. Singular value decomposition-based regression identifies activation of endogenous signaling pathways in vivo. Genome Biol. 2008;9:R180. Figure 5(a)
  • Paraffin embedded magnetically aggregated hepatocyte spheroid section was stained with anti-MRP2 followed by an AF488 conjugated secondary antibody, DAPI, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Desai PK, Tseng H, Souza GR. Assembly of hepatocyte spheroids using magnetic 3D cell culture for CYP450 inhibition/induction. Int J Mol Sci. 2017;18:1085. Figure 2(b)
  • Apoptosis in frozen mouse retinal sections post injection of sham, BENAQ or after DNAse I treatment was detected with terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labeling (TUNEL) and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Tochitsky I, Trautman J, Gallerani N, Malis JG, Kramer RH. Restoring visual function to the blind retina with a potent, safe and long-lasting photoswitch. Sci Rep. 2017;7:45487. Figure 4(a)
  • Whole mount Wirenia argentea larva was stained with anti-acetylated-α-tubulin followed a FITC conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Todt C, Wanninger A. Of tests, trochs, shells, and spicules: Development of the basal mollusk Wirenia argentea (Solenogastres) and its bearing on the evolution of trochozoan larval key features. Front Zool. 2010;7:6. Figure 5(a)
  • Frozen pCMV-eGFP-injected mdx4cv gastrocnemius section was stained with anti-desmin followed by a Texas Red® conjugated secondary antibody and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Guess MG, Barthel KK, Pugach EK, Leinwand LA. Measuring microRNA reporter activity in skeletal muscle using hydrodynamic limb vein injection of plasmid DNA combined with in vivo imaging. Skelet Muscle. 2013;3:19. Figure 5

    Texas Red® is a registered trademark of Thermo Fisher Scientific, Inc.
  • Frozen bitransgenic MTB/TRAS mouse mammary tissue section 96 hr post administration of doxycycline was stained with anti-cytokeratin 8 and anti-Smad4 followed by AF488 and AF567 conjugated secondary antibodies and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Liu Z, Wang M, Alvarez JV, Bonney ME, Chen C, D'Cruz C, et al. Singular value decomposition-based regression identifies activation of endogenous signaling pathways in vivo. Genome Biol. 2008;9:R180. Figure 5(a)
  • Whole mount Hispolia malayensis zooid was stained with an AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Schwaha T, Wood TS, Wanninger A. Myoanatomy and serotonergic nervous system of the ctenostome Hislopia malayensis: evolutionary trends in bodyplan patterning of ectoprocta. Front Zool. 2011;8:11. Figure 4(e)
  • Whole mount Argyrotheca cordata larva was stained with AF488 conjugated phalloidin followed by DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Altenburger A, Wanninger A. Comparative larval myogenesis and adult myoanatomy of the rhynchonelliform (articulate) brachiopods Argyrotheca cordata, A. cistellula, and Terebratalia transversa. Front Zool. 2009;6:3. Figure 3(e)
  • Whole mount Argyrotheca cordata larva was stained with AF488 conjugated phalloidin followed by DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Altenburger A, Wanninger A. Comparative larval myogenesis and adult myoanatomy of the rhynchonelliform (articulate) brachiopods Argyrotheca cordata, A. cistellula, and Terebratalia transversa. Front Zool. 2009;6:3. Figure 3(d)
  • Human normal trabecular meshwork cell line was incubated with DiD labeled non-pigmented ciliary epithelium-derived exosomes for 32 hours followed by anti-α-tubulin and a secondary antibody. The DNA was stained with DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Lerner N, Avissar S, Beit-Yannai E. Extracellular vesicles mediate signaling between the aqueous humor producing and draining cells in the ocular system. PLoS One. 2017;12(2):e0171153. Figure 2(a)
  • EGFP-RhoB transfected HL-60 cells differentiated to granulocytes were incubated with Texas Red® conjugated zymosan A BioParticles® and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Johnson JL, Ellis BA, Munafo DB, Brzezinska AA, Catz SD. Gene transfer and expression in human neutrophils. The phox homology domain of p47phox translocates to the plasma membrane but not to the membrane of mature phagosomes. BMC Immunol. 2006;7:28. Figure 8(b)

    BioParticles® and Texas Red® are registered trademarks of Thermo Fisher Scientific, Inc.
  • Apchu745 mutant zebrafish embryo brain section was stained with anti-BrdU followed by an AF647 conjugated secondary antibody, TO-PRO-3, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Lin J, Wang X, Dorsky RI. Progenitor expansion in apc mutants is mediated by Jak/Stat signaling. BMC Dev Biol. 2011;11:73. Figure 2(c)
  • GFP-prestin/Xpress-tagged Fabp3 co-transfected opossum kidney cells were stained with anti-Xpress followed by an AF546 conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Zheng J, Anderson CT, Miller KK, Cheatham M, Dallos P. Identifying components of the hair-cell interactome involved in cochlear amplification. BMC Genomics. 2009;10:127. Figure 5(c)
  • Paraffin embedded rat anteroventral periventricular nucleus section was stained with anti-GFAP and anti-NeuN followed by AF488 and AF633 secondary antibodies and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Bu L, Lephart ED. AVPV neurons containing estrogen receptor-beta in adult male rats are influenced by soy isoflavones. BMC Neurosci. 2007;8:13. Figure 3(c)
  • Paraffin embedded adult human wild type αSyn transgenic mice brain section post injection with recombinant human WT αSyn fibrils was stained with anti-GFAP and anti-pSer129-αSyn followed by AF488 and AF594 conjugated secondary antibodies, Sudan Black, and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Sorrentino ZA, Brooks MM, Hudson V 3rd, Rutherford NJ, Golde TE, Giasson BI, et al. Intrastriatal injection of α-synuclein can lead to widespread synucleinopathy independent of neuroanatomic connectivity. Mol Neurodegener. 2017;12:40. Figure 8(b)
  • Frozen Pax7+/- mouse esophagus section was stained with anti-sarcomeric actin and anti-α-smooth muscle followed by AF488 and AF568 conjugated antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Chihara D, Romer AI, Bentzinger CF, Rudnicki MA, Krauss RS. PAX7 is required for patterning the esophageal musculature. Skelet Muscle. 2015;5:39. Figure 1(g)
  • Frozen porcine retina section was stained with anti-SV2 followed by an AF488 conjugated secondary antibody, propidium iodide (PI), and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Townes-Anderson E, Wang J, Halász É, Sugino I, Pitler A, Whitehead I, et al. Fasudil, a clinically used ROCK inhibitor, stabilizes rod photoreceptor synapses after retinal detachment. Transl Vis Sci Technol. 2017;6:22. Figure 2(a)
  • Mouse skeletal muscle fibers were stained with AF488 conjugated anti-BTX and anti-neuronal class III β-tubulin followed by an AF568 conjugated secondary antibody and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Mate SE, Van Der Meulen JH, Arya P, Bhattacharyya S, Band H, Hoffman EP. Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction. Skelet Muscle. 2012;2:19. Figure 6(b)
  • Frozen Tas1R3-GFP mouse larynx section was stained with anti-SubP followed by an AF568 secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

    Image from Tizzano M, Cristofoletti M, Sbarbati A, Finger TE. Expression of taste receptors in solitary chemosensory cells of rodent airways. BMC Pulm Med. 2011;11:3. Figure 1(d)

Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G (SB Cat. No. 0100-01).

Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)
 

Zika virus (ZIKV) inoculated frozen mouse placenta was stained with anti-flaviviruses and anti-cytokeratin. The DNA was stained with DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Vermillion MS, Lei J, Shabi Y, Baxter VK, Crilly NP, McLane M, et al. Intrauterine Zika virus infection of pregnant immunocompetent mice models transplacental transmission and adverse perinatal outcomes. Nat Commun. 2017;8:14575. Figure 4(b)
 

Frozen zebrafish retina section was stained with anti-Zpr3 followed by an AF543 secondary antibody and MBODEC and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Watanabe K, Nishimura Y, Oka T, Nomoto T, Kon T, Shintou T, et al. In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neurosci. 2010;11:116. Figure 3(d)
 

Whole mount Ophryotrocha diadema adult was stained with TRITC conjugated phalliodin and anti-acetylated tubulin followed by a CY2 conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Bergter A, Brubacher JL, Paululat A. Muscle formation during embryogenesis of the polychaete Ophryotrocha diadema (Dorvilleidae) - new insights into annelid muscle patterns. Front Zool. 2008;5:1. Figure 2(a)
 

Whole mount Lineus albocinctus pilidium larva was stained with anti-FMRFamide and anti-acetylated- α-tubulin followed by AF568 and AF633 conjugated secondary antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Hindinger S, Schwaha T, Wanninger A. Immunocytochemical studies reveal novel neural structures in nemertean pilidium larvae and provide evidence for incorporation of larval components into the juvenile nervous system. Front Zool. 2013;10:31. Figure 5(a)
 

Optic nerve explant cultures were stained with anti-Stim2 and anti-GPAF followed by secondary antibodies. The DNA was stained with Hoechst and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Papanikolaou M, Lewis A, Butt AM. Store-operated calcium entry is essential for glial calcium signalling in CNS white matter. Brain Struct Funct. 2017 Feb 28. doi:10.1007/s00429-017-1380-8 [Epub ahead of print]. Figure 3(c)
 

Frozen simian immunodeficiency virus infected rhesus macaque ileum section was stained with anti-IL-7 and anti-cytokeratin followed by AF488 and AF546 conjugated secondary antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Ponte R, Rancez M, Figueiredo-Morgado S, Dutrieux J, Fabre-Mersseman V, Charmeteau-de-Muylder B, et al. Acute simian immunodeficiency virus infection triggers early and transient interleukin-7 production in the gut, leading to enhanced local chemokine expression and intestinal immune cell homing. Front Immunol. 2017;8:588. Figure 1(d)
 

Whole mount Symsagittifera roscoffensis embryo was stained with AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool. 2008;5:14. Figure 2(b)
 

pEGP-BDNF transfected human umbilical cord blood-derived mesenchymal stem cells were stained with anti-BDNF followed by a secondary antibody and DAB and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Lim JY, Park SH, Jeong CH, Oh JH, Kim SM, Ryu CH, et al. Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells. BMC Biotechnol. 2010;10:38. Figure 7(a)
 

Twistp-GFP-actin-Vrp1f06715 transfected porcine aortic endothelial cells were stained with TRITC conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Eriksson T, Varshney G, Aspenström P, Palmer RH. Characterisation of the role of Vrp1 in cell fusion during the development of visceral muscle of Drosophila melanogaster. BMC Dev Biol. 2010;10:86. Figure 8(b)
 

Cultured rat retinal ganglion cells were stained with anti-Thy-1 and anti-neurofilament-L followed by secondary antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Pang I, Zeng H, Fleenor DL, Clark AF. Pigment epithelium-derived factor protects retinal ganglion cells. BMC Neurosci. 2007;8:11. Figure 1(d)
 

Whole mount Hispolia malayensis zooid was stained with an AF488 conjugated phalloidin followed by DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Schwaha T, Wood TS, Wanninger A. Myoanatomy and serotonergic nervous system of the ctenostome Hislopia malayensis: evolutionary trends in bodyplan patterning of ectoprocta. Front Zool. 2011;8:11. Figure 3(b)
 

Arabidopsis culture following BrdU incubation was stained with anti-BrdU followed by an AF488 conjugated secondary antibody and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Kotogány E, Dudits D, Horváth GV, Ayaydin F. A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine. Plant Methods. 2010;6:5. Figure 1(b)
 

Human retinal pigmented epithelial cells were stained with 546 labeled phalloidin and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Hua K, Ferland RJ. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5. Figure 1
 

SLICK-H::Ranbp2flox/flox YFP-expressing mouse nerve cyrosections at day10 post-tamoxifen administration were stained with anti-HDAC4 followed by an AF594 conjugated secondary antibody, Hoechst 33342, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Cho K, Yoon D, Qiu S, Danziger Z, Grill WM, Wetsel WC, et al. Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes. Dis Model Mech. 2017;10:559-79. Figure 4(d)
 

MDA-MB-231 cells were stained with conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from McConnell BV, Koto K, Gutierrez-Hartmann A. Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression. Mol Cancer. 2011;10:75. Figure 5
 

Frozen I4895T mouse soleus muscle section was stained with PROTEOSTAT® aggresome detection reagent and Hoechst 33342 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Lee CS, Hanna AD, Wang H, Dagnino-Acosta A, Joshi AD, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659. Figure 6(a)

PROTEOSTAT® is a registered trademark of Enzo Life Sciences, Inc.
 

Human retinal pigmented epithelial cells were stained with anti-β-tubulin followed by an AF488 secondary antibody and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Hua K, Ferland RJ. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5. Figure 1
 

Whole mount Ophryotrocha diadema embryo was stained with TRITC conjugated phalliodin and anti-acetylated tubulin followed by a CY2 conjugated secondary antibody and DRAQ5™ and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Bergter A, Brubacher JL, Paululat A. Muscle formation during embryogenesis of the polychaete Ophryotrocha diadema (Dorvilleidae) - new insights into annelid muscle patterns. Front Zool. 2008;5:1. Figure 5(g)

DRAQ5™ is a registered trademark of Biostatus Limited
 

Frozen mouse circumvallate papillae section was stained with anti-IP3R3 followed by a Rhodamine Red™-X secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Clapp TR, Stone LM, Margolskee RF, Kinnamon SC. Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction. BMC Neurosci. 2001;2:6. Figure 4(b)

Rhodamine Red™ is a trademark of Thermo Fisher Scientific, Inc.
 

Frozen Dcc+/+ mouse lumbar dorsal root ganglion section was stained with anti-CGRP, anti-isolectin B4, and anti-Substance P followed by AF488, AF555, and AF647 conjugated secondary reagents and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Liang D, Zheng M, Sun Y, Sahbaie P, Low SA, Peltz G, et al. The Netrin-1 receptor DCC is a regulator of maladaptive responses to chronic morphine administration. BMC Genomics. 2014;15:345. Figure 5(a)
 

Whole mount Symsagittifera roscoffensis embryo was stained with AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool. 2008;5:14. Figure 3(a)
 

Frozen TRPM5-GFP mouse circumvallate papillae section was stained with propidium iodide and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Vandenbeuch A, Clapp TR, Kinnamon SC. Amiloride-sensitive channels in type I fungiform taste cells in mouse. BMC Neurosci. 2008;9:1. Figure 5
 

Whole mount Drosophila larva testis was stained with anti-DSXM, anti-TJ, and anti-EYA followed by AF488 and AF647 conjugated secondary antibodies and tyramide signal amplification CY3 system and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Hempel LU, Oliver B. Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells. BMC Dev Biol. 2007;7:113. Figure 7(d)
 

Whole S. littoralis ovariole was stained with anti-YP2 followed by an AF594 conjugated secondary antibody, Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Bebas P, Kotwica J, Joachimiak E, Giebultowicz JM. Yolk protein is expressed in the insect testis and interacts with sperm. BMC Dev Biol. 2008;8:64. Figure 4(b)
 

Frozen mouse embryo jugular lymph sac section was stained with anti-Prox1 and anti-LA102 followed by AF488 and AF594 conjuagted secondary antibodies and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Buttler K, Ezaki T, Wilting J. Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics. BMC Dev Biol. 2008;8:43. Figure 1(d)
 

Frozen bitransgenic MTB/TRAS mouse mammary tissue section was stained with anti-cytokeratin 8 and anti-Smad4 followed by AF488 and AF567 conjugated secondary antibodies and Hoechst 33258 then mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Liu Z, Wang M, Alvarez JV, Bonney ME, Chen C, D'Cruz C, et al. Singular value decomposition-based regression identifies activation of endogenous signaling pathways in vivo. Genome Biol. 2008;9:R180. Figure 5(a)
 

Paraffin embedded magnetically aggregated hepatocyte spheroid section was stained with anti-MRP2 followed by an AF488 conjugated secondary antibody, DAPI, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Desai PK, Tseng H, Souza GR. Assembly of hepatocyte spheroids using magnetic 3D cell culture for CYP450 inhibition/induction. Int J Mol Sci. 2017;18:1085. Figure 2(b)
 

Apoptosis in frozen mouse retinal sections post injection of sham, BENAQ or after DNAse I treatment was detected with terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labeling (TUNEL) and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Tochitsky I, Trautman J, Gallerani N, Malis JG, Kramer RH. Restoring visual function to the blind retina with a potent, safe and long-lasting photoswitch. Sci Rep. 2017;7:45487. Figure 4(a)
 

Whole mount Wirenia argentea larva was stained with anti-acetylated-α-tubulin followed a FITC conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Todt C, Wanninger A. Of tests, trochs, shells, and spicules: Development of the basal mollusk Wirenia argentea (Solenogastres) and its bearing on the evolution of trochozoan larval key features. Front Zool. 2010;7:6. Figure 5(a)
 

Frozen pCMV-eGFP-injected mdx4cv gastrocnemius section was stained with anti-desmin followed by a Texas Red® conjugated secondary antibody and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Guess MG, Barthel KK, Pugach EK, Leinwand LA. Measuring microRNA reporter activity in skeletal muscle using hydrodynamic limb vein injection of plasmid DNA combined with in vivo imaging. Skelet Muscle. 2013;3:19. Figure 5

Texas Red® is a registered trademark of Thermo Fisher Scientific, Inc.
 

Frozen bitransgenic MTB/TRAS mouse mammary tissue section 96 hr post administration of doxycycline was stained with anti-cytokeratin 8 and anti-Smad4 followed by AF488 and AF567 conjugated secondary antibodies and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Liu Z, Wang M, Alvarez JV, Bonney ME, Chen C, D'Cruz C, et al. Singular value decomposition-based regression identifies activation of endogenous signaling pathways in vivo. Genome Biol. 2008;9:R180. Figure 5(a)
 

Whole mount Hispolia malayensis zooid was stained with an AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Schwaha T, Wood TS, Wanninger A. Myoanatomy and serotonergic nervous system of the ctenostome Hislopia malayensis: evolutionary trends in bodyplan patterning of ectoprocta. Front Zool. 2011;8:11. Figure 4(e)
 

Whole mount Argyrotheca cordata larva was stained with AF488 conjugated phalloidin followed by DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Altenburger A, Wanninger A. Comparative larval myogenesis and adult myoanatomy of the rhynchonelliform (articulate) brachiopods Argyrotheca cordata, A. cistellula, and Terebratalia transversa. Front Zool. 2009;6:3. Figure 3(e)
 

Whole mount Argyrotheca cordata larva was stained with AF488 conjugated phalloidin followed by DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Altenburger A, Wanninger A. Comparative larval myogenesis and adult myoanatomy of the rhynchonelliform (articulate) brachiopods Argyrotheca cordata, A. cistellula, and Terebratalia transversa. Front Zool. 2009;6:3. Figure 3(d)
 

Human normal trabecular meshwork cell line was incubated with DiD labeled non-pigmented ciliary epithelium-derived exosomes for 32 hours followed by anti-α-tubulin and a secondary antibody. The DNA was stained with DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Lerner N, Avissar S, Beit-Yannai E. Extracellular vesicles mediate signaling between the aqueous humor producing and draining cells in the ocular system. PLoS One. 2017;12(2):e0171153. Figure 2(a)
 

EGFP-RhoB transfected HL-60 cells differentiated to granulocytes were incubated with Texas Red® conjugated zymosan A BioParticles® and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Johnson JL, Ellis BA, Munafo DB, Brzezinska AA, Catz SD. Gene transfer and expression in human neutrophils. The phox homology domain of p47phox translocates to the plasma membrane but not to the membrane of mature phagosomes. BMC Immunol. 2006;7:28. Figure 8(b)

BioParticles® and Texas Red® are registered trademarks of Thermo Fisher Scientific, Inc.
 

Apchu745 mutant zebrafish embryo brain section was stained with anti-BrdU followed by an AF647 conjugated secondary antibody, TO-PRO-3, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Lin J, Wang X, Dorsky RI. Progenitor expansion in apc mutants is mediated by Jak/Stat signaling. BMC Dev Biol. 2011;11:73. Figure 2(c)
 

GFP-prestin/Xpress-tagged Fabp3 co-transfected opossum kidney cells were stained with anti-Xpress followed by an AF546 conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Zheng J, Anderson CT, Miller KK, Cheatham M, Dallos P. Identifying components of the hair-cell interactome involved in cochlear amplification. BMC Genomics. 2009;10:127. Figure 5(c)
 

Paraffin embedded rat anteroventral periventricular nucleus section was stained with anti-GFAP and anti-NeuN followed by AF488 and AF633 secondary antibodies and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Bu L, Lephart ED. AVPV neurons containing estrogen receptor-beta in adult male rats are influenced by soy isoflavones. BMC Neurosci. 2007;8:13. Figure 3(c)
 

Paraffin embedded adult human wild type αSyn transgenic mice brain section post injection with recombinant human WT αSyn fibrils was stained with anti-GFAP and anti-pSer129-αSyn followed by AF488 and AF594 conjugated secondary antibodies, Sudan Black, and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Sorrentino ZA, Brooks MM, Hudson V 3rd, Rutherford NJ, Golde TE, Giasson BI, et al. Intrastriatal injection of α-synuclein can lead to widespread synucleinopathy independent of neuroanatomic connectivity. Mol Neurodegener. 2017;12:40. Figure 8(b)
 

Frozen Pax7+/- mouse esophagus section was stained with anti-sarcomeric actin and anti-α-smooth muscle followed by AF488 and AF568 conjugated antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Chihara D, Romer AI, Bentzinger CF, Rudnicki MA, Krauss RS. PAX7 is required for patterning the esophageal musculature. Skelet Muscle. 2015;5:39. Figure 1(g)
 

Frozen porcine retina section was stained with anti-SV2 followed by an AF488 conjugated secondary antibody, propidium iodide (PI), and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Townes-Anderson E, Wang J, Halász É, Sugino I, Pitler A, Whitehead I, et al. Fasudil, a clinically used ROCK inhibitor, stabilizes rod photoreceptor synapses after retinal detachment. Transl Vis Sci Technol. 2017;6:22. Figure 2(a)
 

Mouse skeletal muscle fibers were stained with AF488 conjugated anti-BTX and anti-neuronal class III β-tubulin followed by an AF568 conjugated secondary antibody and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Mate SE, Van Der Meulen JH, Arya P, Bhattacharyya S, Band H, Hoffman EP. Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction. Skelet Muscle. 2012;2:19. Figure 6(b)
 

Frozen Tas1R3-GFP mouse larynx section was stained with anti-SubP followed by an AF568 secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Image from Tizzano M, Cristofoletti M, Sbarbati A, Finger TE. Expression of taste receptors in solitary chemosensory cells of rodent airways. BMC Pulm Med. 2011;11:3. Figure 1(d)
 
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61. Schaller T, Appel N, Koutsoudakis G, Kallis S, Lohmann V, Pietschmann T, et al. Analysis of hepatitis C virus superinfection exclusion by using novel fluorochrome gene-tagged viral genomes. J Virol. 2007;81:4591-603. (ICC, AF488, DAPI, GFP, RFP)
62. Tekle YI, Anderson OR, Lecky AF. Evidence of parasexual activity in "asexual amoebae" Cochliopodium spp. (Amoebozoa): extensive cellular and nuclear fusion. Protist. 2014;165:676-87. (ICC, AF488, AF594, CellMask™ Orange, Hoechst 33358)
63. Thekkedath R, Koshkaryev A, Torchilin VP. Lysosome-targeted octadecyl-rhodamine B-liposomes enhance lysosomal accumulation of glucocerebrosidase in Gaucher's cells in vitro. Nanomedicine (Lond). 2013;8:1055-65. (ICC, DyLight™ 350, LysoTracker™ Red)
64. Wolins NE, Rubin B, Brasaemle DL. TIP47 associates with lipid droplets. J Biol Chem. 2001;276:5101-8. (ICC, BODIPY™ 493/503)
65. Gauthier LR, Granotier C, Hoffschir F, Etienne O, Ayouaz A, Desmaze C, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012;69:629-40. (FISH, CY3, DAPI, FITC)
66. Chabaud M, Heuzé ML, Bretou M, Vargas P, Maiuri P, Solanes P, et al. Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. Nat Commun. 2015;6:7526. (PLA, AF488)
67. Kruspe M, Thieme H, Guntinas-Lichius O, Irintchev A. Motoneuron regeneration accuracy and recovery of gait after femoral nerve injuries in rats. Neuroscience. 2014;280:73-87. (IHC, Fluoro-Emerald, Fluoro-Ruby®)
68. Lewis MD, Behrends J, Sá E Cunha C, Mendes AM, Lasitschka F, Sattler JM, et al. Chemical attenuation of Plasmodium in the liver modulates severe malaria disease progression. J Immunol. 2015;194:4860-70. (IHC, AF488, AF555, AF660, Hoechst 33258)
69. Lomeli N, Di K, Czerniawski J, Guzowski JF, Bota DA. Cisplatin-induced mitochondrial dysfunction is associated with impaired cognitive function in rats. Free Radic Biol Med. 2017;102:274-86. (IHC, FD Rapid GolgiStain™)
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