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Alexa Fluor® Conjugated Antibodies

Alexa Fluor® dyes are some of the most widely used fluorescent dyes for scientific research owing to their brightness, photostability, and instrument compatibility. They are typically conjugated to primary or secondary antibodies, allowing researchers to perform applications including flow cytometry, immunohistochemistry (IHC), and immunocytochemistry (ICC), as well as fluorescence-linked immunosorbent assay (FLISA) and Western blotting. Because Alexa Fluor® dyes are available in a broad range of colors, they provide numerous options for multiplexed detection.

We offer an extensive selection of Alexa Fluor®-conjugated primary and secondary antibodies, including F(ab)2 and Fab fragments, as well as isotype controls from many different host species. We also provide custom antibody conjugation services, should you need an alternative conjugate of a SouthernBiotech-manufactured antibody or a custom conjugation of your own antibody.

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Frequently Asked Questions

Alexa Fluor® dyes are synthesized through the sulfonation of coumarin, rhodamine, xanthene, and cyanine dyes. Sulfonation not only makes Alexa Fluor® dyes brighter and more photostable than their spectral analogues, but it also introduces pH insensitivity in the 4-10 range. As a result, Alexa Fluor® dyes are well-suited to many different research applications.

Alexa Fluor® dyes are classified according to the approximate excitation wavelength maximum, given in nm. For example, Alexa Fluor® 488 has an excitation maximum of ~490 nm, while Alexa Fluor® 647 has an excitation maximum of ~650 nm.

A major advantage of Alexa Fluor® dyes is that they are brighter than many commonly used spectral analogues. Alexa Fluor® dyes are also more photostable than many other conjugates, allowing more time to capture images in microscopy applications. Other advantages of Alexa Fluor® dyes include their pH stability (Alexa Fluor® dyes are stable at pH 4-10), water solubility (Alexa Fluor®-conjugated antibodies are relatively resistant to precipitation during storage), and emission diversity (Alexa Fluor® dyes have emission spectra spanning blue to near-infrared). In addition, Alexa Fluor® dyes are compatible with the standard excitation sources found in most flow cytometers, fluorescence microscopes, and other instruments used for fluorescence-based detection.

We offer an extensive selection of primary and secondary antibodies, as well as isotype controls, conjugated to Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 647, and Alexa Fluor® 700. We also provide custom antibody conjugation services to give researchers even greater flexibility for experimental design.

When planning a multiplexed experiment, it is critically important to select fluorophores with distinct emission spectra. This is to avoid spectral overlap that could otherwise lead to results being misinterpreted. For example, if your panel already includes a FITC-conjugated antibody (emission maximum 518 nm, green) and an Alexa Fluor® 555-conjugated antibody (emission maximum 580 nm, orange), you might wish to consider using an antibody conjugated to Alexa Fluor® 647 (emission maximum 665 nm, red) or Alexa Fluor® 700 (emission maximum 723 nm, near-infrared) for detecting an additional target.

All SouthernBiotech Alexa Fluor®-conjugated antibodies are quality tested in-house by flow cytometry and/or fluorescence-linked immunosorbent assay (FLISA) to ensure target specificity and lot-to-lot consistency.

Alexa Fluor®-conjugated antibodies should be stored at 2-8°C, avoiding exposure to light. They can also be aliquoted into smaller volumes, to avoid freeze/thaw cycles, and stored at -20°C.

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