Cat. No.
0108-01

Size
10 mg

Price (USD)
$105.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Source	Normal rat serum
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	5.0 mg/mL
Volume	2.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include - Flow Cytometry ^3-9,16 ELISA ^1,2 FLISA Other referenced applications for relevant formats include - Immunohistochemistry-Paraffin Sections ^10,17 Immunohistochemistry-Frozen Sections ¹⁸ Immunocytochemistry ^11,19 Blocking ¹² In vivo Control ^13-15,20,21
Recommended Dilutions	Please refer to product specific Technical Bulletin

ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

References

1.	Doymaz MZ, Rouse BT. Herpetic stromal keratitis: an immunopathologic disease mediated by CD4+ T lymphocytes. Invest Ophthalmol Vis Sci. 1992;33:2165-73. (ELISA)
2.	Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (ELISA)
3.	Garbe A, Buck J, Hämmerling U. Retinoids are important cofactors in T cell activation. J Exp Med. 1992;176:109-17. (FC)
4.	Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC)
5.	Abernethy NJ, Hagan C, Tan PL, Watson JD. Dysregulated expression of CD69 and IL-2 receptor α and β chains on CD8⁺ T lymphocytes in flaky skin mice. Immunol Cell Biol. 2000;78:596-602. (FC)
6.	Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4⁺CD25⁺ human thymocytes. J Exp Med. 2002;196:379-87. (FC)
7.	Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8⁺CD25⁺ thymocytes share phenotypic and functional features with CD4⁺CD25⁺ regulatory thymocytes. Blood. 2003;102:4107-14. (FC)
8.	Welner R, Swett DJ, Pelsue SC. Age-related loss of bone marrow pre-B- and immature B-lymphocytes in the autoimmune-prone flaky skin mutant mice. Autoimmunity. 2005;38:399-408. (FC)
9.	Abbasi S, Shin D, Beaty N, Masiuk M, Chen S, Gonzalez-Garcia I, et al. Characterization of monoclonal antibodies to the plasma cell alloantigen ENPP1. Hybridoma. 2011;30:11-17. (FC)
10.	Almholt K, Lund LR, Rygaard J, Nielsen BS, Danø K, Rømer J, et al. Reduced metastasis of transgenic mammary cancer in urokinase-deficient mice. Int J Cancer. 2005;113:525-32. (IHC-PS)
11.	Lu DP, Tian L, O'Neill C, King NJ. Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos. Cell Res. 2002;12:373-83. (ICC)
12.	Pepin AC, Tandon R, Cattori V, Niederer E, Riond B, Willi B, et al. Cellular segregation of feline leukemia provirus and viral RNA in leukocyte subsets of long-term experimentally infected cats. Virus Res. 2007;127:9-16. (Block)
13.	Broudy VC, Lin NL, Priestley GV, Nocka K, Wolf NS. Interaction of stem cell factor and its receptor c-kit mediates lodgment and acute expansion of hematopoietic cells in the murine spleen. Blood. 1996;88:75-81. (In vivo control)
14.	Cheng M, Nguyen M, Fantuzzi G, Koh TJ. Endogenous interferon-γ is required for efficient skeletal muscle regeneration. Am J Physiol Cell Physiol. 2008;294:C1183-91. (In vivo control)
15.	Ma Y, Zhang X, Bao H, Mi S, Cai W, Yan H, et al. Toll-like receptor (TLR) 2 and TLR4 differentially regulate doxorubicin induced cardiomyopathy in mice. PloS One. 2012;7(7):e40763. (In vivo control)
16.	Mattsson N, Duzevik EG, Pelsue SC. Expansion of CD22^lo B cells in the spleen of autoimmune-prone flaky skin mice. Cell Immunol. 2005;234:124-32. (FC)
17.	Yukami T, Hasegawa M, Matsushita Y, Fujita T, Matsushita T, Horikawa M, et al. Endothelial selectins regulate skin wound healing in cooperation with L-selectin and ICAM-1. J Leukoc Biol. 2007;82:519-31. (IHC-PS)
18.	Yoshizaki A, Iwata Y, Komura K, Ogawa F, Hara T, Muroi E, et al. CD19 regulates skin and lung fibrosis via Toll-like receptor signaling in a model of bleomycin-induced scleroderma. Am J Pathol. 2008;172:1650-63. (IHC-FS)
19.	Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)
20.	Milbauer LC, Enenstein JA, Roney M, Solovey A, Bodempudi V, Nichols TC, et al. Blood outgrowth endothelial cell migration and trapping in vivo: a window into gene therapy. Transl Res. 2009;153:179-89. (In vivo control)
21.	Saino O, Taguchi A, Nakagomi T, Nakano-Doi A, Kashiwamura S, Doe N, et al. Immunodeficiency reduces neural stem/progenitor cell apoptosis and enhances neurogenesis in the cerebral cortex after stroke. J Neurosci Res. 2010;88:2385-97. (In vivo control)

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