Mouse F(ab')2 IgG1-UNLB

Cat. No.
0115-01  
Size
0.5 mg  
Price (USD)
$200.00 
Clone 15H6
Isotype Mouse F(ab')2 IgG1κ
Specificity T-2 mycotoxin
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1
ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Paraffin Sections – Reported in literature 2,3
In vitro Control – Reported in literature 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2793973
  • Human peripheral blood lymphocytes were stained with Mouse F(ab’)2 IgG1-UNLB (SB Cat. No. 0115-01; gray) and Mouse F(ab’)2 Anti-Human CD19-UNLB (SB Cat. No. 9342-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-FITC (SB Cat. No. 1031-02).
  • ELISA plate was coated with serially diluted Mouse F(ab’)2 IgG1-UNLB (SB Cat. No. 0115-01). Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG2b, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG3, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

Human peripheral blood lymphocytes were stained with Mouse F(ab’)2 IgG1-UNLB (SB Cat. No. 0115-01; gray) and Mouse F(ab’)2 Anti-Human CD19-UNLB (SB Cat. No. 9342-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-FITC (SB Cat. No. 1031-02).
 

ELISA plate was coated with serially diluted Mouse F(ab’)2 IgG1-UNLB (SB Cat. No. 0115-01). Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG2b, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG3, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
 
Related Products (6)
References
1. Lund CV, Popkov M, Magnenat L, Barbas CF 3rd. Zinc finger transcription factors designed for bispecific coregulation of ErbB2 and ErbB3 receptors: insights into ErbB receptor biology. Mol Cell Biol. 2005;25:9082-91. (FC)
2. Chatelais L, Jamin A, Gras-Le Guen C, Lallès J, Le Huërou-Luron I, Boudry G. The level of protein in milk formula modifies ileal sensitivity to LPS later in life in a piglet model. PLoS One. 2011;6(5):e19594. (IHC-PS)
3. Boudry G, Jamin A, Chatelais L, Gras-Le Guen C, Michel C, Le Huërou-Luron I. Dietary protein excess during neonatal life alters colonic microbiota and mucosal response to inflammatory mediators later in life in female pigs. J Nutr. 2013;143:1225-32. (IHC-PS)
4. Pilling D, Tucker NM, Gomer RH. Aggregated IgG inhibits the differentiation of human fibrocytes. J Leukoc Biol. 2006;79:1242-51. (In vitro control)