Rat IgG2b-SPRD

Cat. No.
0118-13  
Size
0.1 mg   		Price (USD)
$167.00 
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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone KLH/G2b-1-2
Isotype Rat IgG2bκ
Specificity KLH
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-11,14
ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 12
Immunohistochemistry-Paraffin Sections – Reported in literature 14
In vivo Control – Reported in literature 13
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794020
  • Rat IgG2b-UNLB
    Paraffin embedded AIDS-associated Burkitt lymphoma tissue array was stained with anti-CXCR5 (right) and Rat IgG2b-UNLB (SB Cat. No. 0118-01; left) followed by a secondary antibody and Fast Red.

    Images from Widney DP, Gui D, Popoviciu LM, Said JW, Breen EC, Huang X, et al. Expression and function of the chemokine, CXCL13, and its receptor, CXCR5, in AIDS-associated non-Hodgkin's lymphoma. AIDS Research and Treatment. 2010;2010:164586. Figure 2(a)

Paraffin embedded AIDS-associated Burkitt lymphoma tissue array was stained with anti-CXCR5 (right) and Rat IgG2b-UNLB (SB Cat. No. 0118-01; left) followed by a secondary antibody and Fast Red.

Images from Widney DP, Gui D, Popoviciu LM, Said JW, Breen EC, Huang X, et al. Expression and function of the chemokine, CXCL13, and its receptor, CXCR5, in AIDS-associated non-Hodgkin's lymphoma. AIDS Research and Treatment. 2010;2010:164586. Figure 2(a)
 
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References
1. Frederiksen PD, Thiel S, Larsen CB, Jensenius JC. M-ficolin, an innate immune defence molecule, binds patterns of acetyl groups and activates complement. Scand J Immunol. 2005;62:462-73. (FC)
2. Yamaji T, Mitsuki M, Teranishi T, Hashimoto Y. Characterization of inhibitory signaling motifs of the natural killer cell receptor Siglec-7: attenuated recruitment of phosphatases by the receptor is attributed to two amino acids in the motifs. Glycobiology. 2005;15:667-76. (FC)
3. Chen L, Arora M, Yarlagadda M, Oriss TB, Krishnamoorthy N, Ray A, et al. Distinct responses of lung and spleen dendritic cells to the TLR9 agonist CpG oligodeoxynucleotide. J Immunol. 2006;177:2373-83. (FC)
4. Mor A, Planer D, Luboshits G, Afek A, Metzger S, Chajek-Shaul T, et al. Role of naturally occurring CD4+ CD25+ regulatory T cells in experimental atherosclerosis. Arterioscler Thromb Vasc Biol. 2007;27:893-900. (FC)
5. Mausner-Fainberg K, Luboshits G, Mor A, Maysel-Auslender S, Rubinstein A, Keren G, et al. The effect of HMG-CoA reductase inhibitors on naturally occurring CD4+CD25+ T cells. Atherosclerosis. 2008;197:829-39. (FC)
6. Afek A, Kogan E, Maysel-Auslender S, Mor A, Regev E, Rubinstein A, et al. Clopidogrel attenuates atheroma formation and induces a stable plaque phenotype in apolipoprotein E knockout mice. Microvasc Res. 2009;77:364-9. (FC)
7. Vanden Bush TJ, Buchta CM, Claudio J, Bishop GA. Cutting Edge: Importance of IL-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with B lymphocytes. J Immunol. 2009;183:4833-7. (FC)
8. Lee H, Trott JS, Haque S, McCormick S, Chiorazzi N, Mongini PK. A cyclooxygenase-2/prostaglandin E2 pathway augments activation-induced cytosine deaminase expression within replicating human B cells. J Immunol. 2010;185:5300-14. (FC)
9. Wismar R, Brix S, Lærke HN, Frøkiær H. Comparative analysis of a large panel of non-starch polysaccharides reveals structures with selective regulatory properties in dendritic cells. Mol Nutr Food Res. 2011;55:443-54. (FC)
10. Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, et al. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011;12:151-60. (FC)
11. Koduru S, Wong E, Strowig T, Sundaram R, Zhang L, Strout MP, et al. Dendritic cell-mediated activation-induced cytidine deaminase (AID)-dependent induction of genomic instability in human myeloma. Blood. 2012;119:2302-9. (FC)
12. Løvås T, Bruusgaard JC, Øynebråten I, Gundersen K, Bogen B. DNA vaccines: MHC II-targeted vaccine protein produced by transfected muscle fibres induces a local inflammatory cell infiltrate in mice. PLoS One. 2014;9(10):e108069. (IHC-FS)
13. Gong Y, Koh D. Neutrophils promote inflammatory angiogenesis via release of preformed VEGF in an in vivo corneal model. Cell Tissue Res. 2010;339:437-48. (In vivo control)
14. Widney DP, Gui D, Popoviciu LM, Said JW, Breen EC, Huang X. Expression and function of the chemokine, CXCL13, and its receptor, CXCR5, in AIDS-associated non-Hodgkin's lymphoma. AIDS Research and Treatment. 2010;2010:164586. (FC, IHC-PS)
Spectral Red® is a registered trademark of Southern Biotechnology Associates, Inc.
Spectral Red® is a PE/CY5 tandem conjugate
Cy® is a registered trademark of GE Healthcare