Rat IgM-FITC

Cat. No.
0120-02  
Size
0.5 mg  
Price (USD)
$295.00 
Clone NIP/M-2
Isotype Rat IgMλ
Specificity NIP
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-5
ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Paraffin Sections 6
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with Goat Anti-Rat IgG-UNLB (SB Cat. No. 3030-01) and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgM-FITC (SB Cat. No. 0120-02) was captured and fluorescence intensity quantified.

FLISA plate was coated with Goat Anti-Rat IgG-UNLB (SB Cat. No. 3030-01) and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgM-FITC (SB Cat. No. 0120-02) was captured and fluorescence intensity quantified.
 
References
1. Zhang X, Li W, Wu Y, Gao M. Amelioration of doxorubicin-induced myocardial oxidative stress and immunosuppression by grape seed proanthocyanidins in tumour-bearing mice. J Pharm Pharmacol. 2005;57:1043-51. (FC)
2. Zhang X, Li W, Wu Y, Zheng T, Li W, Qu S, et al. Proanthocyanidin from grape seeds potentiates anti-tumor activity of doxorubicin via immunomodulatory mechanism. Int Immunopharmacol. 2005;5:1247-57. (FC)
3. Chu VT, Enghard P, Riemekasten G, Berek C. In vitro and in vivo activation induces BAFF and APRIL expression in B cells. J Immunol. 2007;179:5947-57. (FC)
4. Chu VT, Enghard P, Schürer S, Steinhauser G, Rudolph B, Riemekasten G, et al. Systemic activation of the immune system induces aberrant BAFF and APRIL expression in B cells in patients with systemic lupus erythematosus. Arthritis Rheum. 2009;60:2083-93. (FC)
5. Silvescu CI, Sackstein R. G-CSF induces membrane expression of a myeloperoxidase glycovariant that operates as an E-selectin ligand on human myeloid cells. Proc Natl Acad Sci USA. 2014;111:10696-701. (FC)
6. SouthernBiotech unpublished data (IHC-FS)