Mouse IgG2c-UNLB

Cat. No.
0122-01  
Size
1.0 mg  
Price (USD)
$270.00 
Clone 6.3
Isotype Mouse IgG2cκ
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested
ELISA – Quality tested 1-7
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Mouse IgG2c-UNLB (SB Cat. No. 0122-01). Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG2c, Human ads-BIOT (SB Cat. No. 1079-08), Goat Anti-Mouse IgG2b, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG3, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

ELISA plate was coated with serially diluted Mouse IgG2c-UNLB (SB Cat. No. 0122-01). Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG2c, Human ads-BIOT (SB Cat. No. 1079-08), Goat Anti-Mouse IgG2b, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG3, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
 
References
1. Haas KM, Watanabe R, Matsushita T, Nakashima H, Ishiura N, Okochi H, et al. Protective and pathogenic roles for B cells during systemic autoimmunity in NZB/W F1 mice. J Immunol. 2010;184:4789-800. (ELISA)
2. Cardilo-Reis L, Gruber S, Schreier SM, Drechsler M, Papac-Milicevic N, Weber C, et al. Interleukin-13 protects from atherosclerosis and modulates plaque composition by skewing the macrophage phenotype. EMBO Mol Med. 2012;4:1072-86. (ELISA)
3. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012;487:249-53. (ELISA)
4. Donius LR, Handy JM, Weis JJ, Weis JH. Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013;191:434-47. (ELISA)
5. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
6. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
7. Pioli PD, Chen X, Weis JJ, Weis JH. Fatal autoimmunity results from the conditional deletion of Snai2 and Snai3. Cell Immunol. 2015;295:1-18. (ELISA)