ELISA plate was coated with serially diluted Goat IgG Fc-UNLB (SB Cat. No. 0130-01). Immunoglobulin was detected with Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.