Human IgG-AP

Cat. No.
0150-04  
Size
1.0 mL   		Price (USD)
$85.00 
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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Source Normal human plasma
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing 0.1% sodium azide, pH 8.0
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-4
Other referenced applications for relevant formats include -
Western Blot 10
Multiplex 5
Microarray 6
In vivo Control 7,8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794076
  • Human IgG-AP
    ELISA plate was coated with Goat Anti-Human IgG-UNLB (SB Cat. No. 2040-01), Goat Anti-Human IgM-UNLB (SB Cat. No. 2020-01), and Goat Anti-Human IgA-UNLB (SB Cat. No. 2050-01). Serially diluted Human IgG-AP (SB Cat. No. 0150-04) was captured and quantified.

ELISA plate was coated with Goat Anti-Human IgG-UNLB (SB Cat. No. 2040-01), Goat Anti-Human IgM-UNLB (SB Cat. No. 2020-01), and Goat Anti-Human IgA-UNLB (SB Cat. No. 2050-01). Serially diluted Human IgG-AP (SB Cat. No. 0150-04) was captured and quantified.
 
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References
1. Huang Z, Phoolcharoen W, Lai H, Piensook K, Cardineau G, Zeitlin L, et al. High-level rapid production of full-size monoclonal antibodies in plants by a single-vector DNA replicon system. Biotechnol Bioeng. 2010;106:9-17. (ELISA)
2. He J, Lai H, Brock C, Chen Q. A novel system for rapid and cost-effective production of detection and diagnostic reagents of West Nile virus in plants. J Biomed Biotechnol. 2012;2012:106783. (ELISA)
3. He J, Peng L, Lai H, Hurtado J, Stahnke J, Chen Q. A plant-produced antigen elicits potent immune responses against West Nile virus in mice. Biomed Res Int. 2014;2014:952865. (ELISA)
4. He J, Lai H, Engle M, Gorlatov S, Gruber C, Steinkellner H, et al. Generation and analysis of novel plant-derived antibody-based therapeutic molecules against West Nile virus. PLoS One. 2014;9(3):e93541. (ELISA)
5. Ravindranath MH, Terasaki PI, Pham T, Jucaud V, Kawakita S. Therapeutic preparations of IVIg contain naturally occurring anti-HLA-E antibodies that react with HLA-Ia (HLA-A/-B/-Cw) alleles. Blood. 2013;121:2013-28. (Multiplex)
6. Friedman J, Alam SM, Shen X, Xia S, Stewart S, Anasti K, et al. Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS One. 2012;7(5):e37648. (Microarray)
7. Stewart KS, Zhou Z, Zweidler-McKay P, Kleinerman ES. Delta-like ligand 4-Notch signaling regulates bone marrow-derived pericyte/vascular smooth muscle cell formation. Blood. 2011;117:719-26. (In vivo control)
8. Halin C, Tobler NE, Vigl B, Brown LF, Detmar M. VEGF-A produced by chronically inflamed tissue induces lymphangiogenesis in draining lymph nodes. Blood. 2007;110:3158-67. (In vivo control)
9. Phoolcharoen W, Bhoo SH, Lai H, Ma J, Arntzen CJ, Chen Q, et al. Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011;9:807-16. (Dynamic light scattering)
10. SouthernBiotech published data (WB)