Chicken IgY-UNLB

Cat. No.
0170-01  
Size
5.0 mg  
Price (USD)
$115.00 
Source Normal chicken yolk
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 5.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
Flow Cytometry 1
ELISA 2-4
Other referenced applications for relevant formats include -
Immunocytochemistry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Chicken IgY-UNLB (SB Cat. No. 0170-01). Immunoglobulin was detected with Goat Anti-Chicken IgY(H+L)-BIOT (SB Cat. No. 6100-08), Mouse Anti-Chicken IgM-BIOT (SB Cat. No. 8300-08), and Mouse Anti-Chicken IgA-BIOT (SB Cat. No. 8330-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

ELISA plate was coated with serially diluted Chicken IgY-UNLB (SB Cat. No. 0170-01). Immunoglobulin was detected with Goat Anti-Chicken IgY(H+L)-BIOT (SB Cat. No. 6100-08), Mouse Anti-Chicken IgM-BIOT (SB Cat. No. 8300-08), and Mouse Anti-Chicken IgA-BIOT (SB Cat. No. 8330-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
 
References
1. Ekert JE, Murray LA, Das AM, Sheng H, Giles-Komar J, Rycyzyn MA. Chemokine (C-C motif) ligand 2 mediates direct and indirect fibrotic responses in human and murine cultured fibrocytes. Fibrogenesis Tissue Repair. 2011;4:23. (FC, ICC)
2. Alnassan AA, Shehata AA, Kotsch M, Schrödl W, Krüger M, Daugschies A, et al. Efficacy of early treatment with toltrazuril in prevention of coccidiosis and necrotic enteritis in chickens. Avian Pathol. 2013;42:482-90. (ELISA)
3. Bangoura B, Alnassan AA, Lendner M, Shehata AA, Krüger M, Daugschies A. Efficacy of an anticoccidial live vaccine in prevention of necrotic enteritis in chickens. Exp Parasitol. 2014;145:125-34. (ELISA)
4. Wiedmer S, Alnassan AA, Volke B, Thabet A, Daugschies A, Lendner M, et al. Passive immunization with Eimeria tenella gametocyte antigen 56 (EtGAM56) specific antibodies and active immunization trial with the epitope containing peptide. Vet Parasitol. 2017;247:100-7. (ELISA)