Mouse Anti-Bcl-2-UNLB

Cat. No.
10065-01  
Size
0.1 mg   		Price (USD)
$180.00 
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Clone 10C4
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Murine Bcl-2 peptide (a.a. 61-76)
Specificity Mouse/Rat Bcl-2
Alternalte Name(s) B cell leukemia 2
Description Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death, or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be membrane-bound since it is detectable in crude membrane and nuclear fractions, but not in the soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2’s ability to promote cell survival.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Western Blot – Quality tested 1-4
Immunoprecipitation – Reported in literature 3,4
Flow Cytometry – Reported in literature 5,6
Immunohistochemistry-Frozen Sections – Reported in literature 7
Immunohistochemistry-Paraffin Sections – Reported in literature 8
Immunocytochemistry – Reported in literature 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794109
Gene ID
12043 (Mouse)
24224 (Rat)
Gene Symbol
Bcl2 (Mouse)
Bcl2 (Rat)
Gene Aliases AW986256; Bcl-2; C430015F12Rik; D630044D05Rik; D830018M01Rik
Uniprot ID
P10417 (Mouse)
P49950 (Rat)
Uniprot Name
BCL2_MOUSE 
BCL2_RAT
  • Mouse Anti-Bcl-2-UNLB
    Mouse thymus tissue lysate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-2-UNLB (SB Cat. No. 10065-01) followed by Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05) and chemiluminescent detection.

Mouse thymus tissue lysate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-2-UNLB (SB Cat. No. 10065-01) followed by Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05) and chemiluminescent detection.
 
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References
1. Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, WB)
2. Hsu Y, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-XL during apoptosis. Proc Natl Acad Sci USA. 1997;94:3668-72. (WB)
3. Conus S, Kaufmann T, Fellay I, Otter I, Rossé T, Borner C. Bcl-2 is a monomeric protein: prevention of homodimerization by structural constraints. EMBO J. 2000;19:1534-44. (WB, IP, ICC)
4. Gaudette BT, Iwakoshi NN, Boise LH. Bcl-xL protein protects from C/EBP homologous protein (CHOP)-dependent apoptosis during plasma cell differentiation. J Biol Chem. 2014;289:23629-40. (WB, IP)
5. McFarland HI, Puig M, Grajkowska LT, Tsuji K, Lee JP, Mason KP, et al. Regulatory T cells in γ irradiation-induced immune suppression. PLoS One. 2012;7(6):e39092. (FC)
6. Wang X, Szymczak-Workman AL, Gravano DM, Workman CJ, Green DR, Vignali DA. Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis. Cell Death Dis. 2012;3:e270. (FC)
7. Yamanaka T, Helgeland L, Farstad IN, Fukushima H, Midtvedt T, Brandtzaeg P. Microbial colonization drives lymphocyte accumulation and differentiation in the follicle-associated epithelium of Peyer's patches. J Immunol. 2003;170:816-22. (IHC-FS)
8. Saatli B, Kizildag S, Posaci C, Dogan E, Koyuncuoglu M, Ulukus EC, et al. Long-term effects of GnRH agonist, GnRH antagonist, and estrogen plus progesterone treatment on apoptosis related genes in rat ovary. Fertil Steril. 2009;91:2006-11. (IHC-PS)