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Mouse Anti-Bcl-2-UNLB

Cat. No.
10065-01

Size
0.1 mg

Price (USD)
$180.00

Qty	Add to Cart

Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	10C4
Isotype	Mouse IgG₁κ
Isotype Control	Mouse IgG₁-UNLB
Immunogen	Murine Bcl-2 peptide (a.a. 61-76)
Specificity	Mouse/Rat Bcl-2
Alternalte Name(s)	B cell leukemia 2
Description	Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death, or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be membrane-bound since it is detectable in crude membrane and nuclear fractions, but not in the soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2’s ability to promote cell survival.
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - Western Blot – Quality tested ^1-4 Immunoprecipitation – Reported in literature ^3,4 Flow Cytometry – Reported in literature ^5,6 Immunohistochemistry-Frozen Sections – Reported in literature ⁷ Immunohistochemistry-Paraffin Sections – Reported in literature ⁸ Immunocytochemistry – Reported in literature ³
Recommended Dilutions	Please refer to product specific Technical Bulletin

References

1.	Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, WB)
2.	Hsu Y, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-X_L during apoptosis. Proc Natl Acad Sci USA. 1997;94:3668-72. (WB)
3.	Conus S, Kaufmann T, Fellay I, Otter I, Rossé T, Borner C. Bcl-2 is a monomeric protein: prevention of homodimerization by structural constraints. EMBO J. 2000;19:1534-44. (WB, IP, ICC)
4.	Gaudette BT, Iwakoshi NN, Boise LH. Bcl-x_L protein protects from C/EBP homologous protein (CHOP)-dependent apoptosis during plasma cell differentiation. J Biol Chem. 2014;289:23629-40. (WB, IP)
5.	McFarland HI, Puig M, Grajkowska LT, Tsuji K, Lee JP, Mason KP, et al. Regulatory T cells in γ irradiation-induced immune suppression. PLoS One. 2012;7(6):e39092. (FC)
6.	Wang X, Szymczak-Workman AL, Gravano DM, Workman CJ, Green DR, Vignali DA. Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis. Cell Death Dis. 2012;3:e270. (FC)
7.	Yamanaka T, Helgeland L, Farstad IN, Fukushima H, Midtvedt T, Brandtzaeg P. Microbial colonization drives lymphocyte accumulation and differentiation in the follicle-associated epithelium of Peyer's patches. J Immunol. 2003;170:816-22. (IHC-FS)
8.	Saatli B, Kizildag S, Posaci C, Dogan E, Koyuncuoglu M, Ulukus EC, et al. Long-term effects of GnRH agonist, GnRH antagonist, and estrogen plus progesterone treatment on apoptosis related genes in rat ovary. Fertil Steril. 2009;91:2006-11. (IHC-PS)

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