Cat. No.
10100-01

Size
0.5 mg

Price (USD)
$200.00

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Technical Bulletin Certificate of
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Clone	JES3-9D7
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-UNLB
Immunogen	COS-expressed human IL-10
Specificity	Human/Viral/Rhesus/Cynomolgus/Sooty Mangabey IL-10
Alternalte Name(s)	Interleukin-10, cytokine synthesis inhibitory factor, CSIF, TGIF, B-TCGF
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Capture – Quality tested ^1-6 ELISpot-Capture – Reported in literature ^6,7 Flow Cytometry – Reported in literature ^8-15 Immunohistochemistry-Frozen Sections – Reported in literature ¹⁶ Immunohistochemistry-Paraffin Sections – Reported in literature ¹⁷ Western Blot – Reported in literature ¹⁸ Neutralization – Reported in literature ^1,18,19 Multiplex-Capture – Reported in literature ²⁰
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794120

Gene ID	3586 (Human) 694931 (Rhesus) 102133450 (Cynomolgus) 105576723 (Sooty Mangabey)
Gene Symbol	IL10 (Human) IL10 (Rhesus) IL10 (Cynomolgus) IL10 (Sooty Mangabey)
Gene Aliases	CSIF; GVHDS; IL-10; IL10A; TGIF; EGK_01722
Uniprot ID	P22301 (Human) P51496 (Rhesus) P79338 (Cynomolgus) P46651 (Sooty Mangabey)
Uniprot Name	IL10_HUMAN IL10_MACMU IL10_MACFA IL10_CERAT

Standard curve generated with Rat Anti-Human IL-10-UNLB (SB Cat. No. 10100-01; Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (SB Cat. No. 10110-08; Clone JES3-12G8) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human IL-10-UNLB (SB Cat. No. 10100-01; Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (SB Cat. No. 10110-08; Clone JES3-12G8) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

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References

1.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
2.	Gotlieb WH, Abrams JS, Watson JM, Velu TJ, Berek JS, Martínez-Maza O. Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine. 1992;4:385-90. (ELISA-Capture, Viral Reactivity)
3.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
4.	Mosmann T. Measurement of mouse and human interleukin 10. Curr Protoc Immunol. 2001;6.14:1-8. (ELISA-Capture)
5.	Ma Y, Thornton S, Duwel LE, Boivin GP, Giannini EH, Leiden JM, et al. Inhibition of collagen-induced arthritis in mice by viral IL-10 gene transfer. J Immunol. 1998;161:1516-24. (ELISA-Capture, Viral Reactivity)
6.	Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4⁺ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISA-Capture, ELISPOT-Capture)
7.	Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
8.	Koshiba M, Rosin DL, Hayashi N, Linden J, Sitkovsky MV. Patterns of A_2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A_2A receptor monoclonal antibodies. Mol Pharmacol. 1999;55:614-24. (FC)
9.	Kaur A, Grant RM, Means RE, McClure H, Feinberg M, Johnson RP. Diverse host responses and outcomes following simian immunodeficiency virus SIVmac239 infection in sooty mangabeys and rhesus macaques. J Virol. 1998;72:9597-611. (FC, Rhesus & Sooty Mangabey Reactivity)
10.	Gauduin M, Glickman RL, Ahmad S, Yilma T, Johnson RP. Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and β-chemokine production. Proc Natl Acad Sci USA. 1999;96:14031-6. (FC, Rhesus Reactivity)
11.	Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4⁺CD25⁺ human thymocytes. J Exp Med. 2002;196:379-87. (FC)
12.	Gansuvd B, Hubbard WJ, Hutchings A, Thomas FT, Goodwin J, Wilson SB, et al. Phenotypic and functional characterization of long-term cultured rhesus macaque spleen-derived NKT cells. J Immunol. 2003;171:2904-11. (FC, Rhesus Reactivity)
13.	Mattila JT, Diedrich CR, Lin PL, Phuah J, Flynn JL. Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation. J Immunol. 2011;186:3527-37. (FC, Cynomolgus Reactivity)
14.	Souza-Lemos C, de-Campos SN, Teva A, Porrozzi R, Grimaldi G Jr. In situ characterization of the granulomatous immune response with time in nonhealing lesional skin of Leishmania braziliensis-infected rhesus macaques (Macaca mulatta). Vet Immunol Immunopathol. 2011;142:147-55. (FC, Rhesus Reactivity)
15.	Lin PL, Rutledge T, Green AM, Bigbee M, Fuhrman C, Klein E, et al. CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques. AIDS Res Hum Retroviruses. 2012;28:1693-702. (FC, Cynomolgus Reactivity)
16.	Melgar S, Yueng MM, Bas A, Forsberg G, Suhr O, Öberg Å, et al. Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis. Clin Exp Immunol. 2003;134:127-37. (IHC-FS)
17.	Smith DR, Kunkel SL, Burdick MD, Wilke CA, Orringer MB, Whyte RI, et al. Production of interleukin-10 by human bronchogenic carcinoma. Am J Pathol. 1994;145:18-25. (IHC-PS)
18.	Brodeur ND, Spencer JV. Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity. Virus Res. 2010;153:265-8. (WB, Neut, Viral Reactivity)
19.	Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506. (Neut)
20.	Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6 (Multiplex-Capture, Cynomolgus Reactivity)

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