Cat. No.
10114-02

Size
100 tests

Price (USD)
$120.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	B27
Isotype	Mouse IgG₁κ
Isotype Control	Mouse IgG₁-FITC
Immunogen	E. coli-expressed human IFN-γ
Specificity	Human/Rhesus/Cynomolgus/Chimpanzee/Pigtail Macaque/African Green Monkey/Sooty Mangabey IFN-γ
Alternalte Name(s)	Interferon-γ, gIFN, IFNG, immune interferon, IIF, type II interferon, T interferon, T cell interferon, mitogen induced interferon, pH2-labile interferon, macrophage-activating factor, MAF
Format/Conjugate	FITC (Fluorescein)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Detection – Quality tested ^1-4,11 Flow Cytometry – Quality tested ^9,10,12-18 ELISpot-Capture – Reported in literature ^5-10 Immunohistochemistry-Frozen Sections – Reported in literature ¹⁹ Immunocytochemistry – Reported in literature ²⁰ Immunoprecipitation – Reported in literature ¹¹ Neutralization – Reported in literature ^1,21 Multiplex-Capture – Reported in literature ²² Note – May be paired with the purified clone A35 (SB Cat. No. 10113-01) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794141

PMA and ionomycin stimulated human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-PE (SB Cat. No. 9515-09) followed by intracellular staining with Mouse Anti-Human IFN-γ-FITC (SB Cat. No. 10114-02).

PMA and ionomycin stimulated human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-PE (SB Cat. No. 9515-09) followed by intracellular staining with Mouse Anti-Human IFN-γ-FITC (SB Cat. No. 10114-02).

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References

1.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
2.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
3.	Lagier B, Lebel B, Bousquet J, Péne J. Different modulation by histamine of IL-4 and interferon-gamma (IFN-γ) release according to the phenotype of human Th0, Th1 and Th2 clones. Clin Exp Immunol. 1997;108:545-51. (ELISA-Detection)
4.	Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression. Am J Pathol. 2004;164:409-17. (ELISA-Detection)
5.	Barouch DH, Kunstman J, Kuroda MJ, Schmitz JE, Santra S, Peyerl FW, et al. Eventual AIDS vaccine failure in a rhesus monkey by viral escape from cytotoxic T lymphocytes. Nature. 2002;415:335-9. (ELISPOT-Capture, Rhesus Reactivity)
6.	Malkevitch N, Rohne D, Pinczewski J, Aldrich K, Kalyanaraman VS, Letvin NL, et al. Evaluation of combination DNA/replication-competent Ad-SIV recombinant immunization regimens in rhesus macaques. AIDS Res Hum Retroviruses. 2004;20:235-44. (ELISPOT-Capture, Rhesus Reactivity)
7.	Shen L, Shen Y, Huang D, Qiu L, Sehgal P, Du GZ, et al. Development of Vγ2Vδ2⁺ T cell responses during active mycobacterial coinfection of simian immunodeficiency virus-infected macaques requires control of viral infection and immune competence of CD4^{+ T cells. J Infect Dis. 2004;190:1438-47. (ELISPOT-Capture, Rhesus & Pigtail Macaque Reactivity)}
8.	Reimann KA, Parker RA, Seaman MS, Beaudry K, Beddall M, Peterson L, et al. Pathogenicity of simian-human immunodeficiency virus SHIV-89.6P and SIVmac is attenuated in cynomolgus macaques and associated with early T-lymphocyte responses. J Virol. 2005;79:8878-85. (ELISPOT-Capture, Cynomolgus & Rhesus Reactivity)
9.	Letvin NL, Rao SS, Dang V, Buzby AP, Korioth-Schmitz B, Dombagoda D, et al. No evidence for consistent virus-specific immunity in simian immunodeficiency virus-exposed, uninfected rhesus monkeys. J Virol. 2007;81:12368-74. (ELISPOT-Capture, FC, Rhesus Reactivity)
10.	Zahn RC, Rett MD, Korioth-Schmitz B, Sun Y, Buzby AP, Goldstein S, et al. Simian immunodeficiency virus (SIV)-specific CD8⁺ T-cell responses in vervet African green monkeys chronically infected with SIVagm. J Virol. 2008;82:11577-88. (ELISPOT-Capture, FC, African Green Monkey & Rhesus Reactivity)
11.	Favre C, Wijdenes J, Cabrillat H, Djossou O, Banchereau J, de Vries JE. Epitope mapping of recombinant human gamma interferon using monoclonal antibodies. Mol Immunol. 1989;26:17-25. (ELISA-Detection, IP)
12.	Sumpter B, Dunham R, Gordon S, Engram J, Hennessy M, Kinter A, et al. Correlates of preserved CD4⁺ T cell homeostasis during natural, nonpathogenic simian immunodeficiency virus infection of sooty mangabeys: implications for AIDS pathogenesis. J Immunol. 2007;178:1680-91. (FC, Sooty Mangabey Reactivity)
13.	De Rose R, Batten CJ, Smith MZ, Fernandez CS, Peut V, Thomson S, et al. Comparative efficacy of subtype AE simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques. J Virol. 2007;81:292-300. (FC, Pigtail Macaque Reactivity)
14.	Liu J, Ewald BA, Lynch DM, Denholtz M, Abbink P, Lemckert AA, et al. Magnitude and phenotype of cellular immune responses elicited by recombinant adenovirus vectors and heterologous prime-boost regimens in rhesus monkeys. J Virol. 2008;82:4844-52. (FC, Rhesus Reactivity)
15.	Mattila JT, Diedrich CR, Lin PL, Phuah J, Flynn JL. Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation. J Immunol. 2011;186:3527-37. (FC, Cynomolgus Reactivity)
16.	Wilks AB, Perry JR, Ehlinger EP, Zahn RC, White R, Gauduin M, et al. High cell-free virus load and robust autologous humoral immune responses in breast milk of simian immunodeficiency virus-infected african green monkeys. J Virol. 2011;85:9517-26. (FC, African Green Monkey Reactivity)
17.	Reeves RK, Evans TI, Fultz PN, Johnson RP. Potential confusion of contaminating CD16⁺ myeloid DCs with anergic CD16+ NK cells in chimpanzees. Eur J Immunol. 2011;41:1070-4. (FC, Chimpanzee Reactivity)
18.	Lin PL, Rutledge T, Green AM, Bigbee M, Fuhrman C, Klein E, et al. CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques. AIDS Res Hum Retroviruses. 2012;28:1693-702. (FC, Cynomolgus Reactivity)
19.	Stary G, Klein I, Brüggen M, Kohlhofer S, Brunner PM, Spazierer D, et al. Host defense mechanisms in secondary syphilitic lesions: a role for IFN-γ-/IL-17-producing CD8⁺ T cells?. Am J Pathol. 2010;177:2421-32. (IHC-FS)
20.	Chemin K, Bohineust A, Dogniaux S, Tourret M, Guégan S, Miro F, et al. Cytokine secretion by CD4⁺ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity. J Immunol. 2012;189:2159-68. (ICC)
21.	Gangur V, Simons FE, Hayglass KT. Human IP-10 selectively promotes dominance of polyclonally activated and environmental antigen-driven IFN-γ over IL-4 responses. FASEB J. 1998;12:705-13. (Neut)
22.	Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)

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