Cat. No.
10116-01

Size
0.5 mg

Price (USD)
$230.00

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Technical Bulletin Certificate of
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Clone	BVD3-1F9
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-UNLB
Immunogen	Yeast-expressed human IL-3
Specificity	Human IL-3
Alternalte Name(s)	Interleukin-3, burst promoting activity, BP/BPA, eosinophil colony stimulating factor, Eo-CSF, hematopoietic cell growth factor, HCGF, mast cell growth factor, MGF, MCGF, multi-colony stimulating factor, multi-CSF, P cell stimulating activity, PCSA
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Detection – Quality tested ^1-4 Flow Cytometry – Reported in literature ^5,6 Immunohistochemistry-Frozen Sections – Reported in literature ^7,8 Western Blot – Reported in literature ¹ Immunoprecipitation – Reported in literature ^1,2 Note – May be paired with the purified clone BVD8-3G11 (SB Cat. No. 10115-01) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin

Standard curve generated with Rat Anti-Human IL-3-UNLB (SB Cat. No. 10115-01; Clone BVD8-3G11) and Rat Anti-Human IL-3-BIOT (SB Cat. No. 10116-08; Clone BVD3-1F9) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human IL-3-UNLB (SB Cat. No. 10115-01; Clone BVD8-3G11) and Rat Anti-Human IL-3-BIOT (SB Cat. No. 10116-08; Clone BVD3-1F9) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

References

1.	Kaushansky K, Shoemaker SG, Broudy VC, Lin NL, Matous JV, Alderman EM, et al. Structure-function relationships of interleukin-3. An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine interleukin-3. J Clin Invest. 1992;90:1879-88. (Immunogen, ELISA-Detection, WB, IP)
2.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, IP)
3.	Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection)
4.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
5.	Giuliani N, Morandi F, Tagliaferri S, Colla S, Bonomini S, Sammarelli G, et al. Interleukin-3 (IL-3) is overexpressed by T lymphocytes in multiple myeloma patients. Blood. 2006;107:841-2. (FC)
6.	Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (FC)
7.	Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
8.	Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)

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