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Rat Anti-Human IL-13-LE/AF

Cat. No.
10125-14  
Size
0.5 mg  
Price (USD)
$300.00 
Qty
Add to Cart
Clone JES10-5A2
Isotype Rat IgG1κ
Isotype Control Mouse IgG1-LE/AF
Immunogen COS-expressed human IL-13
Specificity Human IL-13
Alternalte Name(s) Interleukin-13, NC30
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Supplied in PBS
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at -20°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 2,3
ELISA-Detection – Reported in literature 1
ELISpot-Detection – Reported in literature 4
Flow Cytometry – Reported in literature 5-10
Immunohistochemistry-Frozen Sections – Reported in literature 11-13
Immunocytochemistry – Reported in literature 14
Neutralization – Reported in literature 15

Note – May be paired with the biotinylated clone SB126d (SB Cat. No. 15930-08) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
References
1. Mascart F, Verscheure V, Malfroot A, Hainaut M, Piérard D, Temerman S, et al. Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses. J Immunol. 2003;170:1504-9. (ELISA-Detection)
2. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
3. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Capture)
4. Marotti B, Rocco A, De Colibus P, Compare D, de Nucci G, Staibano S, et al. Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases. Dig Liver Dis. 2008;40:240-7. (ELISPOT-Detection)
5. Gereda JE, Leung DY, Thatayatikom A, Streib JE, Price MR, Klinnert MD, et al. Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma. Lancet. 2000;355:1680-3. (FC)
6. Yamamoto J, Adachi Y, Onoue Y, Adachi YS, Okabe Y, Itazawa T, et al. Differential expression of the chemokine receptors by the Th1- and Th2-type effector populations within circulating CD4+ T cells. J Leukoc Biol. 2000;68:568-74. (FC)
7. Kim CH, Kunkel EJ, Boisvert J, Johnston B, Campbell JJ, Genovese MC, et al. Bonzo/CXCR6 expression defines type 1-polarized T-cell subsets with extralymphoid tissue homing potential. J Clin Invest. 2001;107:595-601. (FC)
8. Iwasaki M, Nagata K, Takano S, Takahashi K, Ishii N, Ikezawa Z. Association of a new-type prostaglandin D2 receptor CRTH2 with circulating T helper 2 cells in patients with atopic dermatitis. J Invest Dermatol. 2002;119:609-16. (FC)
9. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (FC)
10. Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506. (FC)
11. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
12. Sparrelid E, Emanuel D, Fehniger T, Andersson U, Andersson J. Interstitial pneumonitis in bone marrow transplant recipients is associated with local production of TH2-type cytokines and lack of T cell-mediated cytotoxicity. Transplantation. 1997;63:1782-9. (IHC-FS)
13. Knörr C, Amrehn C, Seeberger H, Rosenwald A, Stilgenbauer S, Ott G, et al. Expression of costimulatory molecules in low-grade mucosa-associated lymphoid tissue-type lymphomas in vivo. Am J Pathol. 1999;155:2019-27. (IHC-FS)
14. Dakhama A, Collins ML, Ohnishi H, Goleva E, Leung DY, Alam R, et al. IL-13-producing BLT1-positive CD8 cells are increased in asthma and are associated with airway obstruction. Allergy. 2013;68:666-73. (ICC)
15. Punnonen J, Yssel H, de Vries JE. The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells. J Allergy Clin Immunol. 1997;100:792-801. (Neut)
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