Cat. No.
10127-01

Size
0.5 mg

Price (USD)
$200.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	BVD13-3A5
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-UNLB
Immunogen	E. coli-expressed human G-CSF
Specificity	Human G-CSF
Alternalte Name(s)	Granulocyte-colony stimulating factor, CSF-β, pluripoietin-β, granulocytic neutrophil colony stimulating activity, G-CSA, macrophage-granulocyte inducer-2, MGI-2, CSF3, CSF3OS, C17orf33
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Capture – Quality tested ^1,2 Immunohistochemistry-Frozen Sections – Reported in literature ^3,4 Immunocytochemistry – Reported in literature ⁵ Neutralization – Reported in literature ¹ Note – May be paired with the biotinylated clone BVD11-37G10 (SB Cat. No. 10128-08) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin

Standard curve generated with Rat Anti-Human G-CSF-UNLB (SB Cat. No. 10127-01; Clone BVD13-3A5) and Rat Anti-Human G-CSF-BIOT (SB Cat. No. 10128-08; Clone BVD11-37G10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05

Standard curve generated with Rat Anti-Human G-CSF-UNLB (SB Cat. No. 10127-01; Clone BVD13-3A5) and Rat Anti-Human G-CSF-BIOT (SB Cat. No. 10128-08; Clone BVD11-37G10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05

References

1.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
2.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
3.	Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
4.	Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
5.	Brauner A, Söderhäll M, Jacobson SH, Lundahl J, Andersson U, Andersson J. Escherichia coli-induced expression of IL-1α, IL-1β, IL-6 and IL-8 in normal human renal tubular epithelial cells. Clin Exp Immunol. 2001;124:423-8. (ICC)

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