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Rat Anti-Human G-CSF-UNLB

Cat. No.
10128-01  
Size
0.5 mg  
Price (USD)
$245.00 
Qty
Add to Cart
Clone BVD11-37G10
Isotype Rat IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen E. coli-expressed human G-CSF
Specificity Human G-CSF
Alternalte Name(s) Granulocyte-colony stimulating factor, CSF-β, pluripoietin-β, granulocytic neutrophil colony stimulating activity, G-CSA, macrophage-granulocyte inducer-2, MGI-2, CSF3, CSF3OS, C17orf33
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Detection – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 3,4
Immunocytochemistry – Reported in literature 5
Drug Delivery – Reported in literature 6

Note – May be paired with the purified clone BVD13-3A5 (SB Cat. No. 10127-01) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Human G-CSF-UNLB (SB Cat. No. 10127-01; Clone BVD13-3A5) and Rat Anti-Human G-CSF-BIOT (SB Cat. No. 10128-08; Clone BVD11-37G10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05

Standard curve generated with Rat Anti-Human G-CSF-UNLB (SB Cat. No. 10127-01; Clone BVD13-3A5) and Rat Anti-Human G-CSF-BIOT (SB Cat. No. 10128-08; Clone BVD11-37G10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05
 
References
1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection)
2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
3. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
4. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
5. Brauner A, Söderhäll M, Jacobson SH, Lundahl J, Andersson U, Andersson J. Escherichia coli-induced expression of IL-1α, IL-1β, IL-6 and IL-8 in normal human renal tubular epithelial cells. Clin Exp Immunol. 2001;124:423-8. (ICC)
6. Rubinstein MP, Salem ML, Doedens AL, Moore CJ, Chiuzan C, Rivell GL, et al. G-CSF/anti-G-CSF antibody complexes drive the potent recovery and expansion of CD11b+Gr-1+ myeloid cells without compromising CD8+ T cell immune responses. J Hematol Oncol. 2013;6:75. (Drug Delivery)
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