Goat Anti-Mouse IgM, Human ads-FITC

Cat. No.
1020-02  
Size
1.0 mg  
Price (USD)
$135.00 
Specificity Reacts with the heavy chain of mouse IgM
Source Pooled antisera from goats hyperimmunized with mouse IgM
Cross Adsorption Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgM covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-11
FLISA
Flow Cytometry 4,7,10,13-17
Other referenced applications for relevant formats include -
ELISpot 2,3,5,6,8-10,12
Immunohistochemistry-Frozen Sections 1,7,18-22
Immunohistochemistry-Paraffin Sections 28
Immunocytochemistry 1,13,23
Electron Microscopy 19
Western Blot 14,16,24
Immunoprecipitation 24,29,30
Purification 26
Stimulation 14,25-27
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794198
  • FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02).
  • Frozen mouse lymph node section was stained with Goat IgG-FITC isotype control (SB Cat. No. 0109-02; left) and Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02; right) followed by DAPI.
  • Wdpks1Δ-1 yeast cells treated with enzymes, denaturant, and hot acid were stained with anti-melanin followed by Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02).

    Image from Paolo WF Jr, Dadachova E, Mandal P, Casadevall A, Szaniszlo PJ, Nosanchuk JD. Effects of disrupting the polyketide synthase gene WdPKS1 in Wangiella [Exophiala] dermatitidis on melanin production and resistance to killing by antifungal compounds, enzymatic degradation, and extremes in temperature. BMC Microbiol. 2006;6:55. Figure 4(d)

FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02).
 

Frozen mouse lymph node section was stained with Goat IgG-FITC isotype control (SB Cat. No. 0109-02; left) and Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02; right) followed by DAPI.
 

Wdpks1Δ-1 yeast cells treated with enzymes, denaturant, and hot acid were stained with anti-melanin followed by Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02).

Image from Paolo WF Jr, Dadachova E, Mandal P, Casadevall A, Szaniszlo PJ, Nosanchuk JD. Effects of disrupting the polyketide synthase gene WdPKS1 in Wangiella [Exophiala] dermatitidis on melanin production and resistance to killing by antifungal compounds, enzymatic degradation, and extremes in temperature. BMC Microbiol. 2006;6:55. Figure 4(d)
 
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References
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