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Rat Anti-Mouse IL-10-FITC

Cat. No.
10211-02  
Size
0.1 mg  
Price (USD)
$165.00 
Qty
Add to Cart
Clone JES5-16E3
Isotype Rat IgG2bκ
Isotype Control Mouse IgG2b-FITC
Immunogen E. coli-expressed mouse IL-10
Specificity Mouse IL-10
Alternalte Name(s) Interleukin-10, B cell derived T cell growth factor, B-TCGF, T cell growth inhibitory factor, TGIF, cytokine synthesis inhibitory factor, CSIF
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Detection – Quality tested 2
ELISA-Capture – Quality tested
Flow Cytometry – Quality tested 3-6
ELISpot-Detection – Reported in literature 2
Immunohistochemsitry-Frozen Sections – Reported in literature 4,7-9
Immunocytochemistry – Reported in literature 1
Immunoprecipitation – Reported in literature 1
Neutralization – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • PMA and Ionomycin-stimulated BALB/c mouse splenocytes were intracellularly stained with Rat Anti-Mouse IL-10-FITC (SB Cat. No. 10211-02).

PMA and Ionomycin-stimulated BALB/c mouse splenocytes were intracellularly stained with Rat Anti-Mouse IL-10-FITC (SB Cat. No. 10211-02).
 
References
1. Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (Immunogen, ICC, IP, Neut)
2. Pack CD, Cestra AE, Min B, Legge KL, Li L, Caprio-Young JC, et al. Neonatal exposure to antigen primes the immune system to develop responses in various lymphoid organs and promotes bystander regulation of diverse T cell specificities. J Immunol. 2001;167:4187-95. (ELISA-Detection, ELISPOT-Detection)
3. Groux H, O'Garra A, Bigler M, Rouleau M, Antonenko S, de Vries JE, et al. A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis. Nature. 1997;389:737-42. (FC)
4. Khanna A, Morelli AE, Zhong C, Takayama T, Lu L, Thomson AW. Effects of liver-derived dendritic cell progenitors on Th1- and Th2-like cytokine responses in vitro and in vivo. J Immunol. 2000;164:1346-54. (FC, IHC-FS)
5. Hamada H, Garcia-Hernandez Mde L, Reome JB, Misra SK, Strutt TM, McKinstry KK, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81. (FC)
6. Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83. (FC)
7. Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
8. Martinelli TM, Van Driel IR, Alderuccio F, Gleeson PA, Toh B. Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis. Gastroenterology. 1996;110:1791-802. (IHC-FS)
9. Sallinen K, Veräjänkorva E, Pöllänen P. Expression of antigens involved in the presentation of lipid antigens and induction of clonal anergy in the female reproductive tract. J Reprod Immunol. 1999;46:91-101. (IHC-FS)
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