Cat. No.
10235-14

Size
0.5 mg

Price (USD)
$250.00

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Technical Bulletin Certificate of
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Clone	MP1-22E9
Isotype	Rat IgG_2aκ
Isotype Control	Rat IgG_2a-LE/AF
Immunogen	COS-expressed mouse GM-CSF
Specificity	Mouse GM-CSF
Alternalte Name(s)	Granulocyte/macrophage colony-stimulating factor, GM CSF, CSF-α, pluripoietin-α, eosinophil colony stimulating factor, Eo-CSF, burst promoting activity, BPA
Format/Conjugate	LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation	Phosphate buffered saline, pH 7.4
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	-20°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Capture – Quality tested ^1-4 ELISpot-Capture – Reported in literature ⁵ Flow Cytometry – Reported in literature ⁶ Immunohistochemsitry-Frozen Sections – Reported in literature ^7-10 Immunocytochemistry – Reported in literature ¹ Western Blot – Reported in literature ¹¹ Neutralization – Reported in literature ^1,2,10,12 Multiplex-Capture – Reported in literature ³ Note – May be paired with the biotinylated clone MP1-31G6 (SB Cat. No. 10236-08) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin

Standard curve generated with Rat Anti-Mouse GM-CSF-UNLB (SB Cat. No. 10235-01; Clone MP1-22E9) and Rat Anti-Mouse GM-CSF-BIOT (SB Cat. No. 10236-08; Clone MP1-31G6) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

Standard curve generated with Rat Anti-Mouse GM-CSF-UNLB (SB Cat. No. 10235-01; Clone MP1-22E9) and Rat Anti-Mouse GM-CSF-BIOT (SB Cat. No. 10236-08; Clone MP1-31G6) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

References

1.	Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (Immunogen, ELISA-Capture, ICC, Neut)
2.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
3.	Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Capture, Multiplex-Capture)
4.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
5.	Hofstetter HH, Ibrahim SM, Koczan D, Kruse N, Weishaupt A, Toyka KV, et al. Therapeutic efficacy of IL-17 neutralization in murine experimental autoimmune encephalomyelitis. Cell Immunol. 2005;237:123-30. (ELISPOT-Capture)
6.	Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR. CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006;177:268-79. (FC)
7.	Martinelli TM, Van Driel IR, Alderuccio F, Gleeson PA, Toh B. Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis. Gastroenterology. 1996;110:1791-802. (IHC-FS)
8.	Morris MM, Dyson H, Baker D, Harbige LS, Fazakerley JK, Amor S. Characterization of the cellular and cytokine response in the central nervous system following Semliki Forest virus infection. J Neuroimmunol. 1997;74:185-97. (IHC-FS)
9.	Schön MP, Schön M, Warren HB, Donohue JP, Parker CM. Cutaneous inflammatory disorder in integrin α_E (CD103)-deficient mice. J Immunol. 2000;165:6583-9. (IHC-FS)
10.	Schön M, Denzer D, Kubitza RC, Ruzicka T, Schön MP. Critical role of neutrophils for the generation of psoriasiform skin lesions in flaky skin mice. J Invest Dermatol. 2000;114:976-83. (IHC-FS, Neut)
11.	Chan C, Blazar BR, Eide CR, Kreitman RJ, Vallera DA. A murine cytokine fusion toxin specifically targeting the murine granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor on normal committed bone marrow progenitor cells and GM-CSF-dependent tumor cells. Blood. 1995;86:2732-40. (WB)
12.	Fontt EO, De Baetselier P, Heirman C, Thielemans K, Lucas R, Vray B. Effects of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor alpha on Trypanosoma cruzi trypomastigotes. Infect Immun. 1998;66:2722-7. (Neut)

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Rat Anti-Mouse GM-CSF-LE/AF