Cat. No.
1030-15

Size
1.0 mg

Price (USD)
$145.00

Qty	Add to Cart

Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Specificity	Reacts with the heavy chains of mouse IgG₁, IgG_2a, IgG_2b, IgG_2c, and IgG₃
Source	Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption	Mouse IgM and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification	Affinity chromatography on mouse IgG covalently linked to agarose
Isotype	Goat IgG
Format/Conjugate	CY5 (Cyanine 5)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Concentration	1.0 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include - ELISA ^1-10 FLISA Flow Cytometry ^5,11-13 Other referenced applications for relevant formats include - ELISpot ^1,3-6 Immunohistochemistry-Frozen Sections ^2,14,15 Immunohistochemistry-Paraffin Sections ^16-18 Immunocytochemistry ^19-22 Western Blot ^7,21,23-25 Multiplex ^26,27
Recommended Dilutions	Please refer to product specific Technical Bulletin

FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-CY5 (SB Cat. No. 1030-15).

FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-CY5 (SB Cat. No. 1030-15).

References

1.	van Ginkel FW, Wahl SM, Kearney JF, Kweon M, Fujihashi K, Burrows PD, et al. Partial IgA-deficiency with increased Th2-type cytokines in TGF-β1 knockout mice. J Immunol. 1999;163:1951-7. (ELISA, ELISPOT)
2.	Dunn LA, Upcroft JA, Fowler EV, Matthews BS, Upcroft P. Orally administered Giardia duodenalis extracts enhance an antigen-specific antibody response. Infect Immun. 2001;69:6503-10. (ELISA, IHC-FS)
3.	Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (ELISA, ELISPOT)
4.	Hovden A, Cox RJ, Haaheim LR. Whole influenza virus vaccine is more immunogenic than split influenza virus vaccine and induces primarily an IgG2a response in BALB/c mice. Scand J Immunol. 2005;62:36-44. (ELISA, ELISPOT)
5.	Conway KL, Kuballa P, Khor B, Zhang M, Shi HN, Virgin HW, et al. ATG5 regulates plasma cell differentiation. Autophagy. 2013;9:528-37. (ELISA, ELISPOT, FC)
6.	Koutsonanos DG, Esser ES, McMaster SR, Kalluri P, Lee J, Prausnitz MR, et al. Enhanced immune responses by skin vaccination with influenza subunit vaccine in young hosts. Vaccine. 2015;33:4675-82. (ELISA, ELISPOT)
7.	Yolken RH, Jones-Brando L, Dunigan DD, Kannan G, Dickerson F, Severance E, et al. Chlorovirus ATCV-1 is part of the human oropharyngeal virome and is associated with changes in cognitive functions in humans and mice. Proc Natl Acad Sci USA. 2014;111:16106-11. (ELISA, WB)
8.	Zhou Y, Maharaj PD, Mallajosyula JK, McCormick AA, Kearney CM. In planta production of flock house virus transencapsidated RNA and its potential use as a vaccine. Mol Biotechnol. 2015;57:325-36. (ELISA)
9.	Zhao H, Bauzon F, Bi E, Yu JJ, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809. (ELISA)
10.	Schubert RD, Hu Y, Kumar G, Szeto S, Abraham P, Winderl J, et al. IFN-β treatment requires B cells for efficacy in neuroautoimmunity. J Immunol. 2015;194:2110-6. (ELISA)
11.	Chen J, Yin H, Xu J, Wang Q, Edelblum KL, Sciammas R, et al. Reversing endogenous alloreactive B cell GC responses with anti-CD154 or CTLA-4Ig. Am J Transplant. 2013;13:2280-92. (FC)
12.	Maxwell P, Melendez-Rodríguez F, Matchett KB, Aragones J, Ben-Califa N, Jaekel H, et al. Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol. 2015;168:429-42. (FC)
13.	Brady AM, Spencer BL, Falsey AR, Nahm MH. Blood collection tubes influence serum ficolin-1 and ficolin-2 levels. Clin Vaccine Immunol. 2014;21:51-5. (FC)
14.	Csencsits K, Burrell BE, Lu G, Eichwald EJ, Stahl GL, Bishop DK. The classical complement pathway in transplantation: unanticipated protective effects of C1q and role in inductive antibody therapy. Am J Transplant. 2008;8:1622-30. (IHC-FS)
15.	Sic H, Kraus H, Madl J, Flittner K, von Münchow AL, Pieper K, et al. Sphingosine-1-phosphate receptors control B-cell migration through signaling components associated with primary immunodeficiencies, chronic lymphocytic leukemia, and multiple sclerosis. J Allergy Clin Immunol. 2014;134:420-8. (IHC-FS)
16.	Zheng P, Zhao YX, Zhang AD, Kang C, Chen HC, Jin ML. Pathologic analysis of the brain from Streptococcus suis type 2 experimentally infected pigs. Vet Pathol. 2009;46:531-5. (IHC-PS)
17.	McPhee CG, Sproule TJ, Shin D, Bubier JA, Schott WH, Steinbuck MP, et al. MHC class I family proteins retard systemic lupus erythematosus autoimmunity and B cell lymphomagenesis. J Immunol. 2011;187:4695-704. (IHC-PS)
18.	Vujasinovic T, Pribic J, Kanjer K, Milosevic NT, Tomasevic Z, Milovanovic Z, et al. Gray-level co-occurrence matrix texture analysis of breast tumor images in prognosis of distant metastasis risk. Microsc Microanal. 2015;21:646-54. (IHC-PS)
19.	Sjöberg M, Garoff H. Interactions between the transmembrane segments of the alphavirus E1 and E2 proteins play a role in virus budding and fusion. J Virol. 2003;77:3441-50. (ICC)
20.	Roberts MS, Woods AJ, Dale TC, van der Sluijs P, Norman JC. Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of αvβ3 and α5β1 integrins. Mol Cell Biol. 2004;24:1505-15. (ICC)
21.	Ma Y, Yates J, Liang Y, Lemon SM, Yi M. NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly. J Virol. 2008;82:7624-39. (ICC, WB)
22.	Mia MM, Bank RA. Paracrine factors of human amniotic fluid-derived mesenchymal stem cells show strong anti-fibrotic properties by inhibiting myofibroblast differentiation and collagen synthesis. J Stem Cell Res Ther. 2015;5:5. (ICC)
23.	Friedman J, Alam SM, Shen X, Xia S, Stewart S, Anasti K, et al. Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS One. 2012;7(5):e37648. (WB)
24.	Bender BJ, Coen DM, Strang BL. Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli. J Virol. 2014;88:11738-47. (WB)
25.	Hethorn WR, Ciarlone SL, Filonova I, Rogers JT, Aguirre D, Ramirez RA, et al. Reelin supplementation recovers synaptic plasticity and cognitive deficits in a mouse model for Angelman syndrome. Eur J Neurosci. 2015;41:1372-80. (WB)
26.	Hovden A, Brokstad KA, Major D, Wood J, Haaheim LR, Cox RJ. A pilot study of the immune response to whole inactivated avian influenza H7N1 virus vaccine in mice. Influenza Other Respir Viruses. 2009;3:21-8. (Multiplex)
27.	Brummelman J, Helm K, Hamstra H, van der Ley P, Boog CJ, Han WG, et al. Modulation of the CD4⁺ T cell response after acellular pertussis vaccination in the presence of TLR4 ligation. Vaccine. 2015;33:1483-91. (Multiplex)

Cy^® is a registered trademark of GE Healthcare

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