The Antibody Company - SouthernBiotech
 
   

Goat Anti-Mouse IgG(H+L), Rat ads-HRP

Cat. No.
1034-05  
Size
1.0 mL  
Price (USD)
$165.00 
Qty

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Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Rat immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
FLISA
Flow Cytometry 3
Other referenced applications for relevant formats include -
Immunoblotting 4-6
Immunocytochemistry 7
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Rat ads-TRITC (SB Cat. No. 1034-03) and DAPI.

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Rat ads-TRITC (SB Cat. No. 1034-03) and DAPI.
 
References
1. van Olffen RW. Modulating T cell homeostasis via TNF and TNFR superfamily members: characterization and function of effector & regulatory T cells [dissertation]. Amsterdam (Netherlands): University of Amsterdam, 2009. (ELISA)
2. Shichkin VP. Immune response of IL-4-knockout mice to low-dose immunization with autologous IL-4. WebmedCentral IMMUNOLOGY. 2011;2(12):WMC002553. (ELISA)
3. Krasinskas AM, Eiref SD, McLean AD, Kreisel D, Gelman AE, Popma SH, et al. Replacement of graft-resident donor-type antigen presenting cells alters the tempo and pathogenesis of murine cardiac allograft rejection. Transplantation. 2000;70:514-21. (FC)
4. Perez-Polo JR, Reilly CB, Rea HC. Oxygen resuscitation after hypoxia ischemia stimulates prostaglandin pathway in rat cortex. Int J Dev Neurosci. 2011;29:639-44. (WB)
5. Bronner DN, O'Riordan MX, He Y. Caspase-2 mediates a Brucella abortus RB51-induced hybrid cell death having features of apoptosis and pyroptosis. Front Cell Infect Microbiol. 2013;3:83. (WB)
6. Xu X, Araki K, Li S, Han J, Ye L, Tan WG, et al. Autophagy is essential for effector CD8+ T cell survival and memory formation. Nat Immunol. 2014;15:1152-61. (WB)
7. SouthernBiotech published data (ICC)
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