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  • Goat Anti-Mouse IgG(H+L)-AF555

Goat Anti-Mouse IgG(H+L)-AF555

Cat. No.:
1036-32
Goat Anti-Mouse IgG(H+L)-Alexa Fluor® 555 antibody for use in fluorescent assays.
$115.00
Size Price (USD) Quantity
1.0 mg $115.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption None; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-4
FLISA

RRID Number AB_2794353

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. Xu L, Cao Y, Xie Z, Huang Q, Bai Q, Yang X, et al. The transcription factor TCF-1 initiates the differentiation of T(FH) cells during acute viral infection. Nat Immunol. 2015;16:991-9. (ELISA)
  2. 2. Li B, Li Z, Wang P, Huang Q, Xu L, He R, et al. Mammalian target of rapamycin complex 1 signalling is essential for germinal centre reaction. Immunology. 2017;152:276-86. (ELISA)
  3. 3. Xu L, Huang Q, Wang H, Hao Y, Bai Q, Hu J, et al. The kinase mTORC1 promotes the generation and suppressive function of follicular regulatory T cells. Immunity. 2017;47:538-51. (ELISA)
  4. 4. Wiedmer S, Alnassan AA, Volke B, Thabet A, Daugschies A, Lendner M, et al. Passive immunization with Eimeria tenella gametocyte antigen 56 (EtGAM56) specific antibodies and active immunization trial with the epitope containing peptide. Vet Parasitol. 2017;247:100-7. (ELISA)
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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) and Rabbit IgG-UNLB (SB Cat. No. 0111-01; left) followed by Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF488 (SB Cat. No. 6442-30) and DAPI.
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  4. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  5. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-AF555 (SB Cat. No. 1037-32) and DAPI.
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  6. HCC827 cells were stained with anti-E-cadherin followed by a secondary antibody and mounted with DAPI Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-20).<br/>Image from Narita M, Shimura E, Nagasawa A, Aiuchi T, Suda Y, Hamada Y, et al. Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c. PLoS One. 2017;12(2):e0172115. Figure 4(e)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. Frozen mouse skeletal muscle tissue was stained with anti-myosin heavy chain and anti-myosin heavy chain type I followed by Goat Anti-Mouse IgG, Human ads-AF555 (SB Cat. No. 1030-32) and Goat Anti-Mouse IgM-FITC (SB Cat. No. 1021-02).<br/>Image from Cortez-Toledo O, Schnair C, Sangngern P, Metzger D, Chao LC. Nur77 deletion impairs muscle growth during developmental myogenesis and muscle regeneration in mice. PLoS One. 2017;12(2):e0171268. Figure 3(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 6415-32) and DAPI.
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  9. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Rat ads-AF555 (SB Cat. No. 1034-32) and DAPI.
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  10. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF488 (SB Cat. No. 6425-30).
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  11. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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