ELISA plate was coated with purified mouse IgG, IgM, and IgA and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1037-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).