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  • Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-HRP

Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-HRP

Cat. No.:
1038-05
Goat Anti-Mouse IgG(H+L)-HRP antibody with minimal reactivity to human, rat, hamster, goat, sheep, rabbit, chicken, guinea pig, horse, and bovine serum proteins for use in ELISA and immunocytochemistry assays.
$203.00
Size Price (USD) Quantity
1.0 mL $203.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Human, rat, hamster, goat, sheep, rabbit, chicken, guinea pig, horse, and bovine serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 1
RRID Number AB_2794363

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. Siman R, Shahim P, Tegner Y, Blennow K, Zetterberg H, Smith DH. Serum SNTF increases in concussed professional ice hockey players and relates to the severity of postconcussion symptoms. J Neurotrauma. 2015;32:1294-300. (ELISA)
  2. 2. SouthernBiotech published data (ICC)

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  2. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  3. CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01L) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05) secondary antibody and chemiluminescent detection.
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  5. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6164-05).
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  6. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L), Rat ads-HRP (SB Cat. No. 1034-05).
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  7. Spleen, brain, and lung tissues from wild type (+/+) and homozygous Limk2 (-/-) mutant mice were resolved by electrophoresis, transfered to PVDF membrane, and probed with anti-LIMK2 followed by Goat Anti-Rat IgG(H+L), Mouse ads-HRP (SB Cat. No. 3050-05) and chemiluminescent detection.<br/>Image from Rice DS, Hansen GM, Liu F, Crist MJ, Newhouse MM, Potter D, et al. Keratinocyte migration in the developing eyelid requires LIMK2. PLoS One. 2012;7(10):e47168. Figure 1(c)<br/>Reproduced under the Creative Commons Attribution License
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  8. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Ig, Human ads-UNLB (SB Cat. No. 1010-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  9. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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