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Goat Anti-Mouse Kappa-TXRD

Cat. No.
1050-07  
Size
1.0 mg  
Price (USD)
$200.00 
Qty

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Specificity Reacts with mouse κ light chains
Source Pooled antisera from goats hyperimmunized with mouse κ light chains
Cross Adsorption Mouse λ light chains; may react with κ light chains from other species
Purification Affinity chromatography on mouse κ light chains covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-TXRD
Format/Conjugate TXRD (Texas Red®)
Buffer Formulation Supplied in PBS/NaN3
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-11
FLISA
Flow Cytometry 3,8,12,15,16,26
Other referenced applications for relevant formats include -
ELISpot 2,7,12-14
Immunohistochemistry-Frozen Sections 17
Immunohistochemistry-Paraffin Sections 18,19
Immunocytochemistry 5,20-22
Immunoblotting 1,4,6,8,9,15,26
Immunoprecipitation 23,24
Stimulation 25,26
Surface Plasmon Resonance 27
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07) and DAPI.
  • Frozen section of TEPC 1165 cell line pellet was stained with Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07).

    Image from Wang H, Owens JD, Shih JH, Li M, Bonner RF, Mushinski JF. Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics. 2006;7:97. Figure 1(d)

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07) and DAPI.
 

Frozen section of TEPC 1165 cell line pellet was stained with Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07).

Image from Wang H, Owens JD, Shih JH, Li M, Bonner RF, Mushinski JF. Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics. 2006;7:97. Figure 1(d)
 
References
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3. D'Agnillo P, Levine JS, Subang R, Rauch J. Prothrombin binds to the surface of apoptotic, but not viable, cells and serves as a target of lupus anticoagulant autoantibodies. J Immunol. 2003;170:3408-22. (ELISA, FC)
4. Wiens GD, Owen J. Mapping of neutralizing epitopes on Renibacterium salmoninarum p57 by use of transposon mutagenesis and synthetic peptides. Appl Environ Microbiol. 2005;71:2894-901. (ELISA, WB)
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7. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Iga serine/threonines fine-tune Ig a tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)
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9. Nakanishi K, Narimatsu S, Ichikawa S, Tobisawa Y, Kurohane K, Niwa Y, et al. Production of hybrid-IgG/IgA plantibodies with neutralizing activity against Shiga toxin 1. PLoS One. 2013;8(11):e80712. (ELISA, WB)
10. DiLillo DJ, Tan GS, Palese P, Ravetch JV. Broadly neutralizing hemagglutinin stalk-specific antibodies require FcγR interactions for protection against influenza virus in vivo. Nat Med. 2014;20:143-51. (ELISA)
11. Sang A, Niu H, Cullen J, Choi SC, Zheng YY, Wang H, et al. Activation of rheumatoid factor-specific B cells is antigen dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model. J Immunol. 2014;193:1609-21. (ELISA)
12. Seagal J, Edry E, Keren Z, Leider N, Benny O, Machluf M, et al. A fail-safe mechanism for negative selection of isotype-switched B cell precursors is regulated by the Fas/FasL pathway. J Exp Med. 2003;198:1609-19. (ELISPOT, FC)
13. Sharma S, Campbell AM, Chan J, Schattgen SA, Orlowski GM, Nayar R, et al. Suppression of systemic autoimmunity by the innate immune adaptor STING. Proc Natl Acad Sci USA. 2015;112:E710-7. (ELISPOT)
14. Ratliff M, Alter S, McAvoy K, Frasca D, Wright JA, Zinkel SS, et al. In aged mice, low surrogate light chain promotes pro-B-cell apoptotic resistance, compromises the PreBCR checkpoint, and favors generation of autoreactive, phosphorylcholine-specific B cells. Aging Cell. 2015;14:382-90. (ELISPOT)
15. Rakhmanov M. Analysis of B lymphocyte activation and differentiation by expression profiling [dissertation]. Freiburg (Germany); University of Freiburg: 2005. (FC, WB)
16. Campbell KS, Yusa S, Kikuchi-Maki A, Catina TL. NKp44 triggers NK cell activation through DAP12 association that is not influenced by a putative cytoplasmic inhibitory sequence. J Immunol. 2004;172:899-906. (FC)
17. Harris DP, Vogel P, Wims M, Moberg K, Humphries J, Jhaver KG, et al. Requirement for class II phosphoinositide 3-kinase C2α in maintenance of glomerular structure and function. Mol Cell Biol. 2011;31:63-80. (IHC-FS)
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19. Weinstein JS, Nacionales DC, Lee PY, Kelly-Scumpia KM, Yan X, Scumpia PO, et al. Colocalization of antigen-specific B and T cells within ectopic lymphoid tissue following immunization with exogenous antigen. J Immunmol. 2008;181:3259-67. (IHC-PS)
20. Gollahon KA, Hagman J, Brinster RL, Storb U. Ig λ-producing B cells do not show feedback inhibition of gene rearrangement. J Immunol. 1988;141:2771-80. (ICC)
21. Duchez S, Amin R, Cogné N, Delpy L, Sirac C, Pascal V, et al. Premature replacement of μ with α immunoglobulin chains impairs lymphopoiesis and mucosal homing but promotes plasma cell maturation. Proc Natl Acad Sci USA. 2010;107:3064-9. (ICC)
22. Wang N, Yuan A, Ma J, Deng Z, Xue L. Sexing murine embryos with an indirect immunofluorescence assay using phage antibody B9-Fab against SDM antigen. J Vet Med Sci. 2015;77:711-4. (ICC)
23. Skowronek MH, Hendershot LM, Haas IG. The variable domain of nonassembled Ig light chains determines both their half-life and binding to the chaperone BiP. Proc Natl Acad Sci USA. 1998;95:1574-8. (IP)
24. Otero JH, Lizák B, Feige MJ, Hendershot LM. Dissection of structural and functional requirements that underlie the interaction of ERdj3 protein with substrates in the endoplasmic reticulum. J Biol Chem. 2014;289:27504-12. (IP)
25. Ku P, You M, Bose HR Jr. Role and regulation of Rel/NF-κB activity in anti-immunoglobulin-induced apoptosis in WEHI-231 B lymphoma cells. Cell Signal. 2000;12:245-53. (Stim)
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27. Swearingen CA, Carpenter JW, Siegel R, Brittain IJ, Dotzlaf J, Durham TB, et al. Development of a novel clinical biomarker assay to detect and quantify aggrecanase-generated aggrecan fragments in human synovial fluid, serum and urine. Osteoarthritis Cartilage. 2010;18:1150-8. (Surface Plasmon Resonance)
Texas Red® is a registered trademark of Molecular Probes, Inc.
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