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Mouse Anti-Human ZAP-70-AP

Cat. No.
10650-04  
Size
1.0 mL  
Price (USD)
$130.00 
Qty
Add to Cart
Clone SBZAP
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG2a-AP
Immunogen Human ZAP-70 (a.a. 280-309)
Specificity Human ZAP-70
Alternalte Name(s) Tyrosine-protein kinase ZAP-70, zeta-chain-associated protein kinase 70, Syk-related tyrosine kinase
Description ZAP-70 is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP-70 is normally expressed on T cell and NK cells.
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation Supplied in a stock solution of 50 mM Tris/1 mM MgCl2/50% glycerol, pH 8.0, containing NaN3 as a preservative
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-20
ELISA – Quality tested
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Human peripheral blood was intracellularly stained with Mouse Anti-Human ZAP-70-PE (SB Cat. No. 10650-09L).

Human peripheral blood was intracellularly stained with Mouse Anti-Human ZAP-70-PE (SB Cat. No. 10650-09L).
 
References
1. Shankey TV, Forman M, Scibelli P, Cobb J, Smith CM, Mills R, et al. An optimized whole blood method for flow cytometric measurement of ZAP-70 protein expression in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2006;70:259-69. (FC)
2. Gachard N, Salviat A, Boutet C, Arnoulet C, Durrieu F, Lenormand B, et al. Multicenter study of ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia using an optimized flow cytometry method. Haematologica. 2008;93:215-23. (FC)
3. Tolusso B, De Santis M, Bosello S, Gremese E, Gobessi S, Cuoghi I, et al. Synovial B cells of rheumatoid arthritis express ZAP-70 which increases the survival and correlates with the inflammatory and autoimmune phenotype. Clin Immunol. 2009;131:98-108. (FC)
4. Abousamra NK, El-Din MS, Azmy E. T-cell CD38 expression in B-chronic lymphocytic leukaemia. Hematol Oncol. 2009;27:82-9. (FC)
5. Kern W, Dicker F, Schnittger S, Haferlach C, Haferlach T. Correlation of flow cytometrically determined expression of ZAP-70 using the SBZAP antibody with IgVH mutation status and cytogenetics in 1,229 patients with chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2009;76:385-93. (FC)
6. Chantepie SP, Vaur D, Grunau C, Salaün V, Briand M, Parienti J, et al. ZAP-70 intron1 DNA methylation status: determination by pyrosequencing in B chronic lymphocytic leukemia. Leuk Res. 2010;34:800-8. (FC)
7. Michelutti A, Gremese E, Morassi F, Petricca L, Arena V, Tolusso B, et al. B-cell subsets in the joint compartments of seropositive and seronegative rheumatoid arthritis (RA) and No-RA arthritides express memory markers and ZAP70 and characterize the aggregate pattern irrespectively of the autoantibody status. Mol Med. 2011;17:901-9. (FC)
8. Degheidy HA, Venzon DJ, Farooqui MZ, Abbasi F, Arthur DC, Wilson WH, et al. Improved ZAP-70 assay using two clones, multiple methods of analysis and clinical correlation. Cytometry B Clin Cytom. 2011;80:309-17. (FC)
9. Degheidy HA, Venzon DJ, Farooqui MZ, Abbasi F, Arthur DC, Wilson WH, et al. Methodological comparison of two anti-ZAP-70 antibodies. Cytometry B Clin Cytom. 2011;80:300-8. (FC)
10. El-Hussiny MA, Ibrahim L, Azmy E, Shamaa A. Prognostic significance of serum monoclonal immunoglobulin in B-chronic lymphocytic leukemia. Egypt J Haematol. 2012;37:240-5. (FC)
11. Kern W, Bacher U, Haferlach C, Dicker F, Alpermann T, Schnittger S, et al. Monoclonal B-cell lymphocytosis is closely related to chronic lymphocytic leukaemia and may be better classified as early-stage CLL. Br J Haematol. 2012;157:86-96. (FC)
12. Gremese E, Tolusso B, Fedele AL, Canestri S, Alivernini S, Ferracciolo G. ZAP-70+ B cell subset influences response to B cell depletion therapy and early repopulation in rheumatoid arthritis. J Rheumatol. 2012;39:2276-85. (FC)
13. Kern W, Bacher U, Haferlach C, Alpermann T, Dicker F, Schnittger S, et al. Frequency and prognostic impact of the aberrant CD8 expression in 5,523 patients with chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2012;82:145-50. (FC)
14. Degheidy HA, Venzon DJ, Farooqui MZ, Abbasi F, Arthur DC, Wilson WH, et al. Combined normal donor and CLL: Single tube ZAP-70 analysis. Cytometry B Clin Cytom. 2012;82:67-77. (FC)
15. Rizzo D, Bouvier G, Youlyouz-Marfak I, Guerin E, Trimoreau F, Bordessoule D, et al. T/B ratio does not reflect levels of ZAP70 expression in clonal CLL B-cells due to ZAP70 overexpression in patient T-cells. Cytometry B Clin Cytom. 2013;84:125-32. (FC)
16. Fedele AL, Tolusso B, Gremese E, Bosello SL, Carbonella A, Canestri S, et al. Memory B cell subsets and plasmablasts are lower in early than in long-standing rheumatoid arthritis. BMC Immunol. 2014;15:28. (FC)
17. Borge M, Lenicov FR, Nannini PR, de los Ríos Alicandú MM, Podaza E, Ceballos A, et al. The expression of sphingosine-1 phosphate receptor-1 in chronic lymphocytic leukemia cells is impaired by tumor microenvironmental signals and enhanced by piceatannol and R406. J Immunol. 2014;193:3165-74. (FC)
18. Abousamra NK, El-Din MS, Elzahaf EH, Esmael ME. Ectonucleoside triphosphate diphosphohydrolase-1 (E-NTPDase1/CD39) as a new prognostic marker in chronic lymphocytic leukemia. Leuk Lymphoma. 2015;56:113-9. (FC)
19. Lafarge ST, Li H, Pauls SD, Hou S, Johnston JB, Gibson SB, et al. ZAP70 expression directly promotes chronic lymphocytic leukaemia cell adhesion to bone marrow stromal cells. Br J Haematol. 2015;168:139-42. (FC)
20. Lafarge ST, Hou S, Pauls SD, Johnston JB, Gibson SB, Marshall AJ. Differential expression and function of CD27 in chronic lymphocytic leukemia cells expressing ZAP-70. Leuk Res. 2015;39:773-8. (FC)
21. European Research Initiative on Chronic Lymphocytic Leukemia (ERIC): standardization & harmonization of cytometric analysis of ZAP70. http://www.ericll.org/pages/projects/zap70_staining. Accessed January 16, 2010.
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