Cat. No.
1140-02

Size
0.5 mg

Price (USD)
$195.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	1B4B1
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-FITC
Specificity	Mouse IgM
Format/Conjugate	FITC (Fluorescein)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - Flow Cytometry – Quality tested ^1-6 ELISA – Quality tested ^7-10 FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature ^11-15 Western Blot ¹⁹ ELISpot – Reported in literature ¹⁶ Proximity Ligation Assay – Reported in literature ¹⁷ Surface Plasmon Resonance – Reported in literature ¹⁸
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794627

Gene ID	16019 (Mouse)
Gene Symbol	Ighm (Mouse)
Gene Aliases	AI326478; Igh-6; Igh-M; Igh6; Igm; muH
Uniprot ID	P01872 (Mouse)
Uniprot Name	IGHM_MOUSE

BALB/c mouse splenocytes were stained with Rat Anti-Mouse IgM-FITC (SB Cat. No. 1140-02) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).
FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-FITC (SB Cat. No. 1140-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse IgM-FITC (SB Cat. No. 1140-02) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).

FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-FITC (SB Cat. No. 1140-02).

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References

1.	Arens R, Tesselaar K, Baars PA, van Schijndel GM, Hendriks J, Pals ST, et al. Constitutive CD27/CD70 interaction induces expansion of effector-type T cells and results in IFNγ-mediated B cell depletion. Immunity. 2001;15:801-12. (FC)
2.	Cook AJ, Oganesian L, Harumal P, Basten A, Brink R, Jolly CJ. Reduced switching in SCID B cells is associated with altered somatic mutation of recombined S regions. J Immunol. 2003;171:6556-64. (FC)
3.	Cariappa A, Boboila C, Moran ST, Liu H, Shi HN, Pillai S. The recirculating B cell pool contains two functionally distinct, long-lived, posttransitional, follicular B cell populations. J Immunol. 2007;179:2270-81. (FC)
4.	Szyszko EA, Skarstein K, Jonsson R, Brokstad KA. Distinct phenotypes of plasma cells in spleen and bone marrow of autoimmune NOD.B10.H2b mice. Autoimmunity. 2011;44:415-26. (FC)
5.	Andrews SF, Zhang Q, Lim S, Li L, Lee J, Zheng N, et al. Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity. J Exp Med. 2013;210:125-42. (FC)
6.	Gallagher MP, Shrestha A, Magee JM, Wesemann DR. Detection of true IgE-expressing mouse B lineage cells. J Vis Exp. 2014;94:e52264. (FC)
7.	Qin L, Greenland JR, Moriya C, Cayabyab MJ, Letvin NL. Effects of type I interferons on the adjuvant properties of plasmid granulocyte-macrophage colony-stimulating factor in vivo. J Virol. 2007;81:10606-13. (ELISA)
8.	Parker MJ, Xue S, Alexander JJ, Wasserfall CH, Campbell-Thompson ML, Battaglia M, et al. Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice. Diabetes. 2009;58:2277-84. (ELISA)
9.	Huston MW, van Til NP, Visser TP, Arshad S, Brugman MH, Cattoglio C, et al. Correction of murine SCID-X1 by lentiviral gene therapy using a codon-optimized IL2RG gene and minimal pretransplant conditioning. Mol Ther. 2011;19:1867-77. (ELISA)
10.	Chen M, Hong MJ, Sun H, Wang L, Shi X, Gilbert BE, et al. Essential role for autophagy in the maintenance of immunological memory against influenza infection. Nat Med. 2014;20:503-10. (ELISA)
11.	Amano H, Amano E, Moll T, Marinkovic D, Ibnou-Zekri N, Martinez-Soría E, et al. The Yaa mutation promoting murine lupus causes defective development of marginal zone B cells. J Immunol. 2003;170:2293-301. (IHC-FS)
12.	Rempel SA, Hawley RC, Gutiérrez JA, Mouzon E, Bobbitt KR, Lemke N, et al. Splenic and immune alterations of the Sparc-null mouse accompany a lack of immune response. Genes Immun. 2007;8:262-74. (IHC-FS)
13.	Berretta F, St-Pierre J, Piccirillo CA, Stevenson MM. IL-2 contributes to maintaining a balance between CD4⁺Foxp3⁺ regulatory T cells and effector CD4⁺ T cells required for immune control of blood-stage malaria infection. J Immunol. 2011;186:4862-71. (IHC-FS)
14.	Khoo LH, Thiam CH, Soh SY, Angeli V. Splenic extrafollicular reactions and BM plasma cells sustain IgM response associated with hypercholesterolemia. Eur J Immunol. 2015;45:1300-12. (IHC-FS)
15.	A-Gonzalez N, Guillen JA, Gallardo G, Diaz M, de la Rosa JV, Hernandez IH, et al. The nuclear receptor LXRα controls the functional specialization of splenic macrophages. Nat Immunol. 2013;14:831-9. (IHC-FS)
16.	Mayne CG, Amanna IJ, Nashold FE, Hayes CE. Systemic autoimmunity in BAFF-R-mutant A/WySnJ strain mice. Eur J Immunol. 2008;38:587-98. (ELISPOT)
17.	Kläsener K, Maity PC, Hobeika E, Yang J, Reth M. B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk. Elife. 2014;3:e02069. (Proximity ligation assay)
18.	Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (Surface plasmon resonance)
19.	SouthernBiotech unpublished data (WB)

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