Rat Anti-Mouse CD5-Alexa Fluor® 647

Cat. No.
1547-31  
Size
0.1 mg   		Price (USD)
$192.00 
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Clone 53-7.3
Isotype Rat (LOU/Ws1/M) IgG2aκ
Isotype Control Rat IgG2a-Alexa Fluor® 647
Immunogen Spleen cells or thymocyte membranes
Specificity Mouse CD5
Alternalte Name(s) Lyt-1, Ly-1, Lymphocyte antigen 1, Ly-12, Ly-A, T1, Tp67
Description CD5, also known as Lyt-1, is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunoprecipitation – Reported in literature 1
ELISA – Reported in literature 3
Cytoxicity – Reported in literature 2
Blocking – Reported in literature 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794869
Gene ID
12507 (Mouse)
Gene Symbol
Cd5 (Mouse)
Gene Aliases Ly-1; Ly-12; Ly-A; Lyt-1
Uniprot ID
P13379 (Mouse)
Uniprot Name
CD5_MOUSE 
  • Rat Anti-Mouse CD5-AF647
    BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD5-AF647 (SB Cat. No. 1547-31) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD5-AF647 (SB Cat. No. 1547-31) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
 
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References
1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, IP, FC)
2. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980;152:280-95. (FC, IHC-FS)
3. Luo W, van de Velde H, von Hoegen I, Parnes JR, Thielemans K. Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72). J Immunol. 1992;148:1630-4. (ELISA)
4. Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S. A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5. Int Immunol. 1998;10:1185-96. (Block)
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