Rat Anti-Mouse CD45RB-Alexa Fluor® 647

Cat. No.
1675-31  
Size
0.1 mg  
Price (USD)
$190.00 
Clone C363.16A
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-Alexa Fluor® 647
Immunogen Cloned mouse Th cell line
Specificity Mouse CD45RB
Description CD45RB represents an exon B-dependent form of CD45 glycoprotein. It is expressed at high density on peripheral B cells, the suppressor/cytotoxic subpopulation of T lymphocytes, a subset of helper T cells, most thymocytes, and at low density on macrophages and dendritic cells.
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2,4
Separation – Reported in literature 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795137
Gene ID
19264 (Mouse)
Gene Symbol
Ptprc (Mouse)
Gene Aliases loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Uniprot ID
P06800 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45RB-AF647 (SB Cat. No. 1675-31).
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45RB-AF647 (SB Cat. No. 1675-31) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45RB-AF647 (SB Cat. No. 1675-31).
 

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45RB-AF647 (SB Cat. No. 1675-31) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02).
 
Related Products (3)
References
1. Johnson P, Greenbaum L, Bottomly K, Trowbridge IS. Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. J Exp Med. 1989;169:1179-84. (FC)
2. Hathcock KS, Laszlo G, Dickler HB, Sharrow SO, Johnson P, Trowbridge IS, et al. Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. J Immunol. 1992;148:19-28. (FC)
3. Farber DL, Acuto O, Bottomly K. Differential T cell receptor-mediated signaling in naive and memory CD4 T cells. Eur J Immunol. 1997;27:2094-101. (Sep)
4. Borlado LR, Redondo C, Alvarez B, Jimenez C, Criado LM, Flores J, et al. Increased phosphoinositide 3-kinase activity induces a lymphoproliferative disorder and contributes to tumor generation in vivo. FASEB J. 2000;14:895-903. (FC)
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