Hamster Anti-Mouse CD103-Pacific Blue™
Cat. No.
1810-26
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Size
0.1 mg
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Price (USD)
$190.00
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Clone |
2E7
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Isotype |
Hamster (Armenian) IgG2
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Isotype Control |
Hamster IgG-Pacific Blue™
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Immunogen |
C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
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Specificity |
Mouse CD103
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Alternalte Name(s) |
Integrin αIEL, αE integrin, ITGAE
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Description |
CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis, and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 monoclonal antibody M290. 2E7 is reported to have signal-inducing activity in a redirected lysis assay and to costimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro which runs counter to the usual inhibition of CTL by anti-integrins. This costimulatory activity is also shared by the monoclonal antibody M290. The property of costimulation is not unique to the mouse since the combination of anti-human αIEL plus anti-TCR will produce similar proliferation in human cells with the exception that CD8+ cells are not stimulated.
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Format/Conjugate |
PACBLU (Pacific Blue™)
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Buffer Formulation |
Phosphate buffered saline containing 0.1% sodium azide
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Concentration |
0.5 mg/mL
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Volume |
0.2 mL
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Storage & Handling |
2-8°C; Avoid exposure to light
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 2 Immunoprecipitation – Reported in literature 2 Immunohistochemistry-Frozen Sections – Reported in literature 2 Activation – Reported in literature 3
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795381
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Gene ID |
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Gene Symbol |
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Gene Aliases |
A530055J10; CD103; aM290; alpha-E1; alpha-M290
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Uniprot ID |
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Uniprot Name |
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-
BALB/c mouse mesenteric lymph node cells were stained with Hamster Anti-Mouse CD103-PACBLU (SB Cat. No. 1810-26) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
BALB/c mouse mesenteric lymph node cells were stained with Hamster Anti-Mouse CD103-PACBLU (SB Cat. No. 1810-26) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
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References
1. | Goodman T, Lefrancois L. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989;170:1569-81. (Immunogen) | 2. | Lefrançois L, Barrett TA, Havran WL, Puddington L. Developmental expression of the αIELβ7 integrin on T cell receptor γδ and T cell receptor αβ T cells. Eur J Immunol. 1994;24:635-40. (IP, IHC-FS, FC) | 3. | Müller S, Jungo M, Aichele P, Mueller C. CD5- CD8αβ intestinal intraepithelial lymphocytes (IEL) are induced to express CD5 upon antigen-specific activation: CD5- and CD5+ CD8αβ IEL do not represent separate T cell lineages. Eur J Immunol. 1997;27:1756-61. (Activ) |
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Pacific Blue™ is a trademark of Life Technologies Corporation
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