Rat Anti-Mouse CD104-LE/AF

Cat. No.
1855-14  
Size
0.5 mg  
Price (USD)
$270.00 
Clone 346-11A
Isotype Rat (Fischer 344) IgG2aκ
Isotype Control Rat IgG2a-LE/AF
Immunogen Tumor-associated antigen TSP-180 purified from a transplantable BALB/c carcinoma
Specificity Mouse CD104
Alternalte Name(s) Integrin β4
Description CD104 represents the integrin β4 subunit that associates non-covalently with integrin α6 (CD49f) to form the α6β4 integrin heterodimer. The α6β4 complex is expressed on the basal surface of a variety of epithelial cell types particularly on stratified squamous epithelia. It is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes. It also has been found to be associated with a number of malignant tissues. Hemidesmosomal α6β4 plays an important role in the adhesion of epithelial cells to basement membranes via interactions with laminin and/or kalinin anchoring filaments. It is not known if the monoclonal antibody 346-11A can block this adhesion.
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Immunohistochemistry-Frozen Sections – Quality tested 3,5
Immunocytochemistry – Reported in literature 6,7
Flow Cytometry – Reported in literature 6,7
Immunoprecipitation – Reported in literature 1-4,7
Western Blot – Reported in literature 2,3,5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795410
Gene ID
192897 (Mouse)
Gene Symbol
Itgb4 (Mouse)
Gene Aliases AA407042; C230078O20; CD104
Uniprot ID
A2A863 (Mouse)
Uniprot Name
  • Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.

Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
 
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References
1. Kennel SJ, Foote LJ, Flynn KM. Tumor antigen on benign adenomas and on murine lung carcinomas quantitated by a two-site monoclonal antibody assay. Cancer Res. 1986;46:707-12. (Immunogen, IP)
2. Kennel SJ, Foote LJ, Falcioni R, Sonnenberg A, Stringer CD, Crouse C, et al. Analysis of the tumor-associated antigen TSP-180. Identity with α64 in the integrin superfamily. J Biol Chem. 1989;264:15515-21. (IP, WB)
3. Sonnenberg A, Linders CJ. The α6β1 (VLA-6) and α6β4 protein complexes: tissue distribution and biochemical properties. J Cell Sci. 1990;96:207-17. (IP, WB, IHC-FS)
4. Gimond C, Baudoin C, van der Neut R, Kramer D, Calafat J, Sonnenberg A. Cre-loxP-mediated inactivation of the α6A integrin splice variant in vivo: evidence for a specific functional role of α6A in lymphocyte migration but not in heart development. J Cell Biol. 1998;143:253-66. (IP)
5. Mokkapati S, Fleger-Weckmann A, Bechtel M, Koch M, Breitkreutz D, Mayer U, et al. Basement membrane deposition of nidogen 1 but not nidogen 2 requires the nidogen binding module of the laminin γ1 chain. J Biol Chem. 2011;286:1911-8. (WB, IHC-FS)
6. Bagutti C, Wobus AM, Fässler R, Watt FM. Differentiation of embryonal stem cells into keratinocytes: comparison of wild-type and β1 integrin-deficient cells. Dev Biol. 1996;179:184-96. (FC, ICC)
7. Witkowski CM, Bowden GT, Nagle RB, Cress AE. Altered surface expression and increased turnover of the α6β4 integrin in an undifferentiated carcinoma. Carcinogenesis. 2000;21:325-30. (IP, FC, ICC)