Goat F(ab')2 Anti-Human Ig-UNLB

Cat. No.
2012-01  
Size
0.5 mg   		Price (USD)
$55.00 
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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Specificity Reacts with the heavy and light chains of human IgG, IgM, and IgA
Source Pepsin digest of Goat Anti-Human Ig (SB Cat. No. 2010)
Cross Adsorption None; may react with immunoglobulins from other species
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2,5
FLISA
Flow Cytometry 7,8
Other referenced applications for relevant formats include -
ELISpot 3,4
Immunohistochemistry-Frozen Sections 6
Stimulation 9,10
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795572
  • Goat F(ab')2 Anti-Human Ig-UNLB
    ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human Ig-UNLB (SB Cat. No. 2012-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).

ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human Ig-UNLB (SB Cat. No. 2012-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
 
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References
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7. Heile JM, Fong Y, Rosa D, Berger K, Saletti G, Campagnoli S, et al. Evaluation of hepatitis C virus glycoprotein E2 for vaccine design: an endoplasmic reticulum-retained recombinant protein is superior to secreted recombinant protein and DNA-based vaccine candidates. J Virol. 2000;74:6885-92. (FC)
8. Bao G, Li Y, Ma Q, He X, Xing J, Yang X, et al. Isolating human antibody against human hepatocellular carcinoma by guided-selection. Cancer Biol Ther. 2005;4:1374-80. (FC)
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10. Lyubchenko T, Zerbe GO. B cell receptor signaling-based index as a biomarker for the loss of peripheral immune tolerance in autoreactive B cells in rheumatoid arthritis. PLoS One. 2014;9(7):e102128. (Stim)