Mouse Anti-Rat Kappa-BIOT

Cat. No.
3090-08  
Size
0.5 mg  
Price (USD)
$290.00 
Clone K4F5
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen Rat κ hybridoma
Specificity Rat Kappa
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1
ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 2
Western Blot 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795912
Gene ID
500183 (Rat)
Gene Symbol
Igkc (Rat)
Gene Aliases Igk@; D4Mit8
  • ELISA plate was coated with purified rat IgG1κ, IgG2aλ, IgG2aλ, IgG2bκ, IgG2bλ, IgG2cκ, IgMκ, and IgMλ. Immunoglobulins were detected with serially diluted Mouse Anti-Rat Kappa-BIOT (SB Cat. No. 3090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD22-UNLB (SB Cat. No. 1580-01) followed by Mouse Anti-Rat Kappa-BIOT (SB Cat. No. 3090-08) and Streptavidin-FITC (SB Cat. No. 7100-02).

ELISA plate was coated with purified rat IgG1κ, IgG2aλ, IgG2aλ, IgG2bκ, IgG2bλ, IgG2cκ, IgMκ, and IgMλ. Immunoglobulins were detected with serially diluted Mouse Anti-Rat Kappa-BIOT (SB Cat. No. 3090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD22-UNLB (SB Cat. No. 1580-01) followed by Mouse Anti-Rat Kappa-BIOT (SB Cat. No. 3090-08) and Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Letterio J, Rudikoff E, Voong N, Bauer SR. Transforming growth factor-β1 sensitivity is altered in Abl-Myc- and Raf-Myc-induced mouse pre-B-cell tumors. Stem Cells. 2006;24:2611-7. (FC)
2. Park H, Light A, Lahoud MH, Caminschi I, Tarlinton DM, Shortman K. Evolution of B cell responses to Clec9A-targeted antigen. J Immunol. 2013;191:4919-25. (IHC-FS)
3. SouthernBiotech unpublished data (WB)