SBA Clonotyping System-AP

Cat. No.
5300-04  
Size
1 kit   		Price (USD)
$445.00 
Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Description The SBA Clonotyping System-AP kit is designed for the isotyping of mouse monoclonal antibodies. It contains 2.5 mg of capture antibody and 1.0 mL of AP conjugated anti-mouse Ig, mouse IgA, mouse IgG1, mouse IgG2a, mouse IgG2b, mouse IgG3, mouse IgM, mouse κ, mouse λ, and pNPP substrate. The kit may also be utilized for quantitative studies of mouse immunoglobulins in samples such as serum, supernatant, and ascites when used in conjunction with the Mouse Immunoglobulin Panel (SB Cat. No. 5300-01).
Kit Components Goat Anti-Mouse Ig, Human ads-UNLB - 2.5 mg in 1.0 mL
Goat Anti-Mouse Ig, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgA-AP - 1.0 mL
Goat Anti-Mouse IgG1, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgG2a, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgG2b, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgG3, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgM, Human ads-AP - 1.0 mL
Goat Anti-Mouse Kappa-AP - 1.0 mL
Goat Anti-Mouse Lambda-AP - 1.0 mL
pNPP Substrate Tablets - 20 x 5 mg
Buffer Formulation UNLB antibody - Borate buffered saline, pH 8.2
AP conjugates - 50 mM Tris/1 mM MgCl2/50% Glycerol containing 0.1% sodium azide, pH 8.0
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested 1-29
ELISPOT – Reported in literature 16,27,30,31
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796078
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References
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17. Shankar M, Nixon JC, Maier S, Workman J, Farris AD, Webb CF. Anti-nuclear antibody production and autoimmunity in transgenic mice that overexpress the transcription factor Bright. J Immunol. 2007;178:2996-3006. (ELISA - Serum)
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22. Savino MT, Ortensi B, Ferro M, Ulivieri C, Fanigliulo D, Paccagnini E, et al. Rai acts as a negative regulator of autoimmunity by inhibiting antigen receptor signaling and lymphocyte activation. J Immunol. 2009;182:301-8. (ELISA - Serum)
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26. Webb CF, Bryant J, Popowski M, Allred L, Kim D, Harriss J, et al. The ARID family transcription factor bright is required for both hematopoietic stem cell and B lineage development. Mol Cell Biol. 2011;31:1041-53. (ELISA - Serum)
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30. Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni PA, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30. (ELISPOT)
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