AP Mouse Clonotyping System

Cat. No.
5300-04

Size
1 kit

Price (USD)
$445.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Description	The SBA Clonotyping System-AP kit is designed for the isotyping of mouse monoclonal antibodies. It contains 2.5 mg of capture antibody and 1.0 mL of AP conjugated anti-mouse Ig, mouse IgA, mouse IgG₁, mouse IgG_2a, mouse IgG_2b, mouse IgG₃, mouse IgM, mouse κ, mouse λ, and pNPP substrate. The kit may also be utilized for quantitative studies of mouse immunoglobulins in samples such as serum, supernatant, and ascites when used in conjunction with the Mouse Immunoglobulin Panel (SB Cat. No. 5300-01).
Kit Components	• Goat Anti-Mouse Ig, Human ads-UNLB • Goat Anti-Mouse Ig, Human ads-AP • Goat Anti-Mouse IgA-AP • Goat Anti-Mouse IgG₁, Human ads-AP • Goat Anti-Mouse IgG_2a, Human ads-AP • Goat Anti-Mouse IgG_2b, Human ads-AP • Goat Anti-Mouse IgG₃, Human ads-AP • Goat Anti-Mouse IgM, Human ads-AP • Goat Anti-Mouse Kappa-AP • Goat Anti-Mouse Lambda-AP • pNPP Substrate Tablets
Buffer Formulation	UNLB antibody - Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added AP conjugates - Supplied in a stock solution 50 mM Tris/1 mM MgCl₂/50% glycerol, pH 8.0, containing NaN₃ as preservative
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	ELISA – Quality tested ^1-29 ELISPOT – Reported in literature ^16,27,30,31
Recommended Dilutions	Please refer to product specific Technical Bulletin

References

1.	Bergen HR 3^rd, Losman MJ, O'Connor T, Zacharias W, Larson JE, Accavitti MA, et al. Specificity of monoclonal anti-Z-DNA antibodies from unimmunized MRL/Mp-lpr/lpr mice. J Immunol. 1987;139:743-8. (ELISA - Isotyping)
2.	Hrdličková R, Nehyba J, Humphries EH. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J Virol. 1994;68:308-19. (ELISA - Isotyping)
3.	Hong JS, Engler JA. Domains required for assembly of adenovirus type 2 fiber trimers. J Virol. 1996;70:7071-8. (ELISA - Isotyping)
4.	Beier RC, Ripley LH, Young CR, Kaiser CM. Production, characterization, and cross-reactivity studies of monoclonal antibodies against the coccidiostat nicarbazin. J Agric Food Chem. 2001;49:4542-52. (ELISA - Isotyping)
5.	Itoh T, Yoshida M, Chiba T, Kita T, Wakatsuki Y. A coordinated cytotoxic effect of IFN-γ and cross-reactive antibodies in the pathogenesis of Helicobacter pylori gastritis. Helicobacter. 2003;8:268-78. (ELISA - Isotyping)
6.	Beelen R, Boyd B, Garavello JC, Pavanelli GC, Ainsworth AJ. Generation, characterisation and applicability of a monoclonal antibody to hybrid surubim catfish Pseudoplatystoma corruscans (Agassiz) × Pseudoplatystoma fasciatum (Linnaeus) immunoglobulin. Comp Clin Path. 2004;12:191-8. (ELISA - Isotyping)
7.	Moore-Lai D, Rowland E. Discovery and characterization of an antibody, anti-egressin, that is able to inhibit Trypanosoma cruzi egress in vitro. J Parasitol. 2004;90:524-30. (ELISA - Isotyping)
8.	Beier RC, Creemer LC, Ziprin RL, Nisbet DJ. Production and characterization of monoclonal antibodies against the antibiotic tilmicosin. J Agric Food Chem. 2005;53:9679-88. (ELISA - Isotyping)
9.	Liu J, Liu B, Cao Z, Inoue S, Morita K, Tian K, et al. Characterization and application of monoclonal antibodies specific to West Nile virus envelope protein. J Virol Methods. 2008;154:20-6. (ELISA - Isotyping)
10.	Marsh MB, Rice CD. Development, characterization, and technical applications of a fish lysozyme-specific monoclonal antibody (mAb M24-2). Comp Immunol Microbiol Infect Dis. 2010;33:e15-23. (ELISA - Isotyping)
11.	Schreeder DM, Cannon JP, Wu J, Li R, Shakhmatov MA, Davis RS. Cutting edge: FcR-like 6 is an MHC class II receptor. J Immunol. 2010;185:23-7. (ELISA - Isotyping)
12.	Merrell K, Wells S, Henderson A, Gorman J, Alt F, Stall A, et al. The absence of the transcription activator TFE3 impairs activation of B cells in vivo. Mol Cell Biol. 1997;17:3335-44. (ELISA - Serum & Supernatant)
13.	Test ST, Mitsuyoshi J, Connolly CC, Lucas AH. Increased immunogenicity and induction of class switching by conjugation of complement C3d to pneumococcal serotype 14 capsular polysaccharide. Infect Immun. 2001;69:3031-40 (ELISA - Serum)
14.	Schmidt CS, Liu J, Zhang T, Song HY, Sandusky G, Mintze K, et al. Enhanced B cell expansion, survival, and humoral responses by targeting death receptor 6. J Exp Med. 2003;197:51-62. (ELISA - Serum)
15.	Test ST, Mitsuyoshi JK, Hu Y. Depletion of complement has distinct effects on the primary and secondary antibody responses to a conjugate of pneumococcal serotype 14 capsular polysaccharide and a T-cell-dependent protein carrier. Infect Immun. 2005;73:277-86. (ELISA - Serum)
16.	Shimoda M, Li T, Pihkala JP, Koni PA. Role of MHC class II on memory B cells in post-germinal center B cell homeostasis and memory response. J Immunol. 2006;176:2122-33. (ELISA - Serum, ELISPOT)
17.	Shankar M, Nixon JC, Maier S, Workman J, Farris AD, Webb CF. Anti-nuclear antibody production and autoimmunity in transgenic mice that overexpress the transcription factor Bright. J Immunol. 2007;178:2996-3006. (ELISA - Serum)
18.	Nixon JC, Ferrell S, Miner C, Oldham AL, Hochgeschwender U, Webb CF. Transgenic mice expressing dominant-negative bright exhibit defects in B1 B cells. J Immunol. 2008;181:6913-22. (ELISA - Serum)
19.	Bynoté KK, Hackenberg JM, Korach KS, Lubahn DB, Lane PH, Gould KA. Estrogen receptor-α deficiency attenuates autoimmune disease in (NZB x NZW)F₁ mice. Genes Immun. 2008;9:137-52. (ELISA - Serum)
20.	Finetti F, Pellegrini M, Ulivieri C, Savino MT, Paccagnini E, Ginanneschi C, et al. The proapoptotic and antimitogenic protein p66SHC acts as a negative regulator of lymphocyte activation and autoimmunity. Blood. 2008;111:5017-27. (ELISA - Serum)
21.	Weant AE, Michalek RD, Khan IU, Holbrook BC, Willingham MC, Grayson JM. Apoptosis regulators Bim and Fas function concurrently to control autoimmunity and CD8⁺ T cell contraction. Immunity. 2008;28:218-30. (ELISA - Serum)
22.	Savino MT, Ortensi B, Ferro M, Ulivieri C, Fanigliulo D, Paccagnini E, et al. Rai acts as a negative regulator of autoimmunity by inhibiting antigen receptor signaling and lymphocyte activation. J Immunol. 2009;182:301-8. (ELISA - Serum)
23.	Hinman RM, Nichols WA, Diaz TM, Gallardo TD, Castrillon DH, Satterthwaite AB. Foxo3-/- mice demonstrate reduced numbers of pre-B and recirculating B cells but normal splenic B cell sub-population distribution. Int Immunol. 2009;21:831-42. (ELISA - Serum)
24.	Cash H, Relle M, Menke J, Brochhausen C, Jones SA, Topley N, et al. Interleukin 6 (IL-6) deficiency delays lupus nephritis in MRL-Fas^lpr mice: the IL-6 pathway as a new therapeutic target in treatment of autoimmune kidney disease in systemic lupus erythematosus. J Rheumatol. 2010;37:60-70. (ELISA - Serum)
25.	Ohnmacht C, Schwartz C, Panzer M, Schiedewitz I, Naumann R, Voehringer D. Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths. Immunity. 2010;33:364-74. (ELISA - Serum)
26.	Webb CF, Bryant J, Popowski M, Allred L, Kim D, Harriss J, et al. The ARID family transcription factor bright is required for both hematopoietic stem cell and B lineage development. Mol Cell Biol. 2011;31:1041-53. (ELISA - Serum)
27.	Anginot A, Espeli M, Chasson L, Mancini SJ, Schiff C. Galectin 1 modulates plasma cell homeostasis and regulates the humoral immune response. J Immunol. 2013;190:5526-33. (ELISA - Serum & Supernatant, ELISPOT)
28.	Yoachim SD, Nuxoll JS, Bynoté KK, Gould KA. Estrogen receptor alpha signaling promotes Sle1-induced loss of tolerance and immune cell activation and is responsible for sex bias in B6.Sle1 congenic mice. Clin Immunol. 2015;158:153-66. (ELISA - Serum)
29.	Guillaume V, Contamin H, Loth P, Grosjean I, Courbot MC, Deubel V, et al. Antibody prophylaxis and therapy against Nipah virus infection in hamsters. J Virol. 2006;80:1972-8. (ELISA - Ascites)
30.	Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni PA, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30. (ELISPOT)
31.	Park KS, Bayles I, Szlachta-McGinn A, Paul J, Boiko J, Santos P, et al. Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response. J Immunol. 2014;193:4663-74. (ELISPOT)

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SBA Clonotyping System-AP