Rabbit F(ab')2 Anti-Human IgG(H+L)-AP

Cat. No.
6000-04  
Size
1.0 mL  
Price (USD)
$125.00 
Specificity Reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA
Source Pepsin digest of Rabbit Anti-Human IgG(H+L) (SB Cat. No. 6140)
Cross Adsorption None; may react with immunoglobulins from other species
Isotype Rabbit F(ab')2 IgG
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Other referenced applications for relevant formats include -
Immunocytochemistry 4
Western Blot 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796084
  • ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit F(ab')2 Anti-Human IgG(H+L)-AP (SB Cat. No. 6000-04).

ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit F(ab')2 Anti-Human IgG(H+L)-AP (SB Cat. No. 6000-04).
 
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References
1. Fernandéz-Shaw S, Kennedy SH, Hicks BR, Edmonds K, Starkey PM, Barlow DH. Anti-endometrial antibodies in women measured by an enzyme-linked immunosorbent assay. Hum Reprod. 1996;11:1180-4. (ELISA)
2. Sugahara KN, Teesalu T, Larmali PP, Kotamraju VR, Agemy L, Greenwald DR, et al. Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs. Science. 2010;328:1031-5. (ELISA)
3. Janko C, Franz S, Munoz LE, Siebig S, Winkler S, Schett G, et al. CRP/anti-CRP antibodies assembly on the surfaces of cell remnants switches their phagocytic clearance toward inflammation. Front Immunol. 2011;2:70. (ELISA)
4. Marchand LS, St-Hilaire S, Putman EA, Serve KM, Pfau JC. Mesothelial cell and anti-nuclear autoantibodies associated with pleural abnormalities in an asbestos exposed population of Libby MT. Toxicol Lett. 2012;208:168-73. (ICC)
5. Merlo A, Tenca C, Fais F, Battini L, Ciccone E, Grossi CE, et al. Inhibitory receptors CD85j, LAIR-1, and CD152 down-regulate immunoglobulin and cytokine production by human B lymphocytes. Clin Diagn Lab Immunol. 2005;12:705-12. (WB)