Goat Anti-Canine IgG(H+L)-Alexa Fluor® 488

Cat. No.
6070-30  
Size
1.0 mg  
Price (USD)
$125.00 
Specificity Reacts with the heavy and light chains of canine IgG
Source Pooled antisera from goats hyperimmunized with canine IgG
Cross Adsorption None; may react with immunoglobulins from other species and the light chains of other canine immunoglobulins
Purification Affinity chromatography on canine IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-Alexa Fluor® 488
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 3
Immunocytochemistry 4
Western Blot 5-8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796149
  • FLISA plate was coated with purified canine IgG. Immunoglobulin was detected with Goat Anti-Canine IgG(H+L)-AF488 (SB Cat. No. 6070-30).

FLISA plate was coated with purified canine IgG. Immunoglobulin was detected with Goat Anti-Canine IgG(H+L)-AF488 (SB Cat. No. 6070-30).
 
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References
1. Kakkis E, Lester T, Yang R, Tanaka C, Anand V, Lemontt J, et al. Successful induction of immune tolerance to enzyme replacement therapy in canine mucopolysaccharidosis I. Proc Natl Acad Sci USA. 2004;101:829-34. (ELISA)
2. Dickson P, Peinovich M, McEntee M, Lester T, Le S, Krieger A, et al. Immune tolerance improves the efficacy of enzyme replacement therapy in canine mucopolysaccharidosis I. J Clin Invest. 2008;118:2868-76. (ELISA)
3. Wiberg ME, Saari SA, Westermarck E, Meri S. Cellular and humoral immune responses in atrophic lymphocytic pancreatitis in German shepherd dogs and rough-coated collies. Vet Immunol Immunopathol. 2000;76:103-15. (IHC-FS)
4. Ulchar I, Celeska I, Stefanovska J, Jakimovska A. Hematological and biochemical parameters in symptomatic and asymptomatic leishmania-seropositive dogs. Mac Vet Rev. 2015;38:i-viii. (ICC)
5. Mathis A, Åkerstedt J, Tharaldsen J, Ødegaard Ø, Deplazes P. Isolates of Encephalitozoon cuniculi from farmed blue foxes (Alopex lagopus) from Norway differ from isolates from Swiss domestic rabbits (Oryctolagus cuniculus). Parasitol Res. 1996;82:727-30. (WB)
6. Rasmussen UB, Benchaibi M, Meyer V, Schlesinger Y, Schughart K. Novel human gene transfer vectors: evaluation of wild-type and recombinant animal adenoviruses in human-derived cells. Hum Gene Ther. 1999;10:2587-99. (WB)
7. Müller-Doblies UU, Herzog K, Tanner I, Mathis A, Deplazes P. First isolation and characterisation of Encephalitozoon cuniculi from a free-ranging rat (Rattus norvegicus). Vet Parasitol. 2002;107:279-85. (WB)
8. Burk SV. Detection of antibodies against Parascaris equorum excretory-secretory antigens [dissertation]. Lexington (USA): University of Kentucky, 2013 (WB)
Alexa Fluor® is a registered trademark of Life Technologies Corporation