Goat Anti-Feline IgG(H+L)-AP

Cat. No.
6080-04  
Size
1.0 mL  
Price (USD)
$105.00 
Specificity Reacts with the heavy and light chains of feline IgG
Source Pooled antisera from goats hyperimmunized with feline IgG
Cross Adsorption None; may react with immunoglobulins from other species and the light chains of other feline immunoglobulins
Purification Affinity chromatography on feline IgG covalently linked to agarose
Isotype Goat IgG
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-4
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 5,6
Immunoprecipitation 7
Western Blot 8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796152
  • ELISA plate was coated with purified feline IgG. Immunoglobulin was detected with Goat Anti-Feline IgG(H+L)-AP (SB Cat. No. 6080-04).

ELISA plate was coated with purified feline IgG. Immunoglobulin was detected with Goat Anti-Feline IgG(H+L)-AP (SB Cat. No. 6080-04).
 
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References
1. Flynn JN, Cannon CA, Lawrence CE, Jarrett O. Polyclonal B-cell activation in cats infected with feline immunodeficiency virus. Immunology. 1994;81:626-30. (ELISA)
2. Lerner DL, Wagaman PC, Phillips TR, Prospero-Garcia O, Henriksen SJ, Fox HS, et al. Increased mutation frequency of feline immunodeficiency virus lacking functional deoxyuridine-triphosphatase. Proc Natl Acad Sci USA. 1995;92:7480-4. (ELISA)
3. Rigby MA, Hosie MJ, Willett BJ, Mackay N, McDonald M, Cannon C, et al. Comparative efficiency of feline immunodeficiency virus infection by DNA inoculation. AIDS Res Hum Retroviruses. 1997;13:405-12. (ELISA)
4. Armua-Fernandez MT, Castro OF, Crampet A, Bartzabal Á, Hofmann-Lehmann R, Grimm F, et al. First case of peritoneal cystic echinococcosis in a domestic cat caused by Echinococcus granulosus sensu stricto (genotype 1) associated to feline immunodeficiency virus infection. Parasitol Int. 2014;63:300-2. (ELISA)
5. Barr MC, Huitron-Resendiz S, Selway DR, Henriksen SJ, Phillips TR. Exogenous glucocorticoids alter parameters of early feline immunodeficiency virus infection. J Infect Dis. 2000;181:576-86. (FC)
6. Barr MC, Billaud J, Selway DR, Huitron-Resendiz S, Osborn KG, Henriksen SJ, et al. Effects of multiple acute morphine exposures on feline immunodeficiency virus disease progression. J Infect Dis. 2000;182:725-32. (FC)
7. Nishii N, Takasu M, Kojima M, Hachisu T, Wakabayashi K, Iwasawa A, et al. Presence of anti-insulin natural autoantibodies in healthy cats and its interference with immunoassay for serum insulin concentrations. Domest Anim Endocrinol. 2010;38:138-45. (IP)
8. Lerner DL, Grant CK, de Parseval A, Elder JH. FIV infection of IL-2-dependent and -independent feline lymphocyte lines: host cells range distinctions and specific cytokine upregulation. Vet Immunol Immunopathol. 1998;65:277-97. (WB)