Mouse Anti-Goat IgG Fc-Alexa Fluor® 488

Cat. No.
6158-30  
Size
0.1 mg  
Price (USD)
$250.00 
Clone SB115d
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-Alexa Fluor® 488
Immunogen Pooled goat IgG
Specificity Goat IgG Fc
Minimal reactivity to goat IgG Fab and sheep, rabbit, human, monkey, rat, hamster, canine, and swine IgG
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
FLISA – Quality tested
Western Blot – Reported in literature 2
Immunohistochemistry-Frozen Sections 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796224
  • BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD19-UNLB (SB Cat. No. 1576-01) followed by Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) and Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
  • FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
  • CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa, Mouse ads-UNLB (SB Cat. 2061-01) and Goat F(ab’)2 Anti-Human Lambda, Mouse ads-PE (SB Cat. No. 2073-09) followed by Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG1, Human ads-UNLB (SB Cat. No. 1070-01) and Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).

BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD19-UNLB (SB Cat. No. 1576-01) followed by Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) and Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
 

FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
 

CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa, Mouse ads-UNLB (SB Cat. 2061-01) and Goat F(ab’)2 Anti-Human Lambda, Mouse ads-PE (SB Cat. No. 2073-09) followed by Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
 

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG1, Human ads-UNLB (SB Cat. No. 1070-01) and Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
 
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References
1. Tang J, Sun X, Yang H, Zhang B, Li Z, Lin Z, et al. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies. Anal Chim Acta. 2013;776:74-8. (ELISA)
2. Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang H, Locksley RM. Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. Mucosal Immunol. 2016;9:275-86. (WB)
3. SouthernBiotech published data (IHC-FS)
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