Rabbit Anti-Mouse IgG(H+L)-Alexa Fluor® 488

Cat. No.
6170-30  
Size
1.0 mg  
Price (USD)
$125.00 
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pooled antisera from rabbits hyperimmunized with mouse IgG
Cross Adsorption None; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Rabbit IgG
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-4
FLISA
Other referenced applications for relevant formats include -
ELISpot 5
Immunohistochemistry-Frozen Sections 6,7
Immunoprecipitation 8
Western Blot 9-11
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796245
  • FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Mouse IgG(H+L)-AF488 (SB Cat. No. 6170-30).

FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Mouse IgG(H+L)-AF488 (SB Cat. No. 6170-30).
 
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References
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10. Duan Y, Huang S, Yang J, Niu P, Gong Z, Liu X, et al. HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo[a]pyrene and interacts with casein kinase 2. Cell Stress Chaperones. 2014;19:271-9. (WB)
11. Hadzhieva M, Vassilev TL, Roumenina LT, Bayry J, Kaveri SV, Lacroix-Desmazes S, et al. Mechanism and functional implications of the heme-induced binding promiscuity of IgE. Biochemistry. 2015;54:2061-72. (WB)
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