Rabbit Anti-Rat IgG(H+L), Human ads-BIOT

Cat. No.
6185-08  
Size
1.0 mg  
Price (USD)
$105.00 
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from rabbits hyperimmunized with rat IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
Purification Affinity chromatography on rat IgG covalently linked to agarose
Isotype Rabbit IgG
Isotype Control Rabbit IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 2,3
Immunohistochemistry-Paraffin Sections 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796263
  • Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Rat Anti-Mouse IgA-UNLB (SB Cat. No. 1165-01) followed by Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G® Anti-Fade (SB Cat. No. 0100-35).
  • ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Rat Anti-Mouse IgA-UNLB (SB Cat. No. 1165-01) followed by Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08), Streptavidin-CY3.5 (SB Cat. No. 7100-24), DAPI, and mounted with Fluoromount-G® Anti-Fade (SB Cat. No. 0100-35).

Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Rat Anti-Mouse IgA-UNLB (SB Cat. No. 1165-01) followed by Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G® Anti-Fade (SB Cat. No. 0100-35).
 

ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Rat Anti-Mouse IgA-UNLB (SB Cat. No. 1165-01) followed by Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08), Streptavidin-CY3.5 (SB Cat. No. 7100-24), DAPI, and mounted with Fluoromount-G® Anti-Fade (SB Cat. No. 0100-35).
 
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References
1. Novitzky N, Davison G, Abdulla R, Mowla S. Definition of the variables affecting efficacy of immunodepletion ex vivo of peripheral blood progenitor cell grafts by alemtuzumab (Campath in the bag). Biol Blood Marrow Transplant. 2013;19:1753-9. (ELISA)
2. Fang Y, Xu C, Fu Y, Holers VM, Molina H. Expression of complement receptors 1 and 2 on follicular dendritic cells is necessary for the generation of a strong antigen-specific IgG response. J Immunol. 1998;160:5273-9. (IHC-FS)
3. Mao D, Wu X, Deppong C, Friend LD, Dolecki G, Nelson DM, et al. Negligible role of antibodies and C5 in pregnancy loss associated exclusively with C3-dependent mechanisms through complement alternative pathway. Immunity. 2003;19:813-22. (IHC-FS)
4. SouthernBiotech published data (IHC-PS)