Donkey Anti-Mouse IgG(H+L), Human SP ads-CY5

Cat. No.
6410-15  
Size
1.0 mg  
Price (USD)
$155.00 
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pooled antisera from donkeys hyperimmunized with mouse IgG
Cross Adsorption Human serum proteins (SP); may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate CY5 (Cyanine 5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 5
Immunohistochemistry-Frozen Sections 3,4
Immunocytochemistry 6
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Donkey Anti-Mouse IgG(H+L), Human SP ads-CY5 (SB Cat. No. 6410-15).

FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Donkey Anti-Mouse IgG(H+L), Human SP ads-CY5 (SB Cat. No. 6410-15).
 
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References
1. Graham KL, Thibault DL, Steinman JB, Okeke L, Kao PN, Utz PJ. Granzyme B is dispensable for immunologic tolerance to self in a murine model of systemic lupus erythematosus. Arthritis Rheum. 2005;52:1684-93. (ELISA)
2. Parker MJ, Xue S, Alexander JJ, Wasserfall CH, Campbell-Thompson ML, Battaglia M, et al. Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice. Diabetes. 2009;58:2277-84. (ELISA)
3. Broekema M, Harmsen MC, van Luyn MJ, Koerts JA, Petersen AH, van Kooten TG, et al. Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. J Am Soc Nephrol. 2007;18:165-75. (IHC-FS)
4. Li Y, Qin X, Kuang F, Wu R, Duan X, Ju G, et al. Alterations of Fc gamma receptor I and Toll-like receptor 4 mediate the antiinflammatory actions of microglia and astrocytes after adrenaline-induced blood-brain barrier opening in rats. J Neurosci Res. 2008;86:3556-65. (IHC-FS)
5. SouthernBiotech unpublished data (FC)
6. SouthernBiotech published data (ICC)
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